20.500.12556/DiRROS-30530 Association of asthma and anti-asthmatic therapy with oral health and microbiota alterations in children - a cross-sectional study Association of asthma and anti-asthmatic therapy with oral health and microbiota alterations in children Background: Asthma and its treatments are recognized as risk factors for oral health in children. The composition of oral microbiota is linked to asthma, as well as to the development of caries and other oral conditions. This study aimed to assess the impact of asthma and anti-asthmatic therapy on children`s oral health and explore the role of salivary microbiota in this relationship. Methods: A cross-sectional study was conducted, including 100 children with asthma, aged 6 to 18 years, and 50 matched healthy controls. Oral health was assessed using the decayed, missing and filled teeth (DMFT/dft) index, the simplified oral hygiene index (OHI-S) to evaluate dental plaque and calculus, and the gingival index. Saliva flow rate and pH were measured, and the salivary microbiota composition was analyzed using 16 S amplicon sequencing. Results: Children with asthma had a median DMFT/dft index of 4, versus 0 in controls (p < 0.01), a median OHI-S of 0.9 versus 0 (p < 0.01) and a median gingival index of 1 versus 0 (p < 0.01). Daily use of inhaled corticosteroids (ICS) was associated with a higher median OHI-S (1 vs. 0.8, p < 0.01) but had no effect on the DMFT/dft or gingival index. The median unstimulated saliva flow rate and pH were 1.3 mL and 6.25 in children with asthma compared to 2.5 mL and 7.00 in controls (p < 0.01 for both). Children with asthma daily using ICS had a lower saliva flow (1.0 mL) and pH (6.25) compared to non-ICS users (1.5 ml and 6.75, p < 0.01 for both). The salivary microbiota composition in children with asthma differed significantly from controls (p < 0.01), showing increased abundances of Streptococcus, Gemella, and Streptobacillus. Alpha diversity was lower in children with asthma, with reduced richness (p < 0.01) and evenness (p = 0.012). However, no significant associations were identified between salivary microbiota and specific oral health indicators. Conclusions: Asthma negatively affects children`s oral health, including reduced salivary flow, and is associated with alterations in the oral microbiota. However, the development of caries in children with asthma cannot be attributed solely to medications or microbiota alterations. Therefore, meticulous oral hygiene and other preventive measures are especially important in children with asthma. asthma anti-asthmatic agents child microbiota oral health saliva astma zdravila proti astmi otrok mikrobiota ustno zdravje slina true false true Angleški jezik Slovenski jezik Neznano 2026-06-29 09:12:20 2026-06-29 09:12:20 2026-06-30 04:12:06 0000-00-00 00:00:00 2026 0 0 Soavtorji: Boris Egić, Anja Pintaric Lonzaric, Maja Rupnik, Aleksander Mahnic; Opis vira z dne 20. 3. 2026 10 str. št. članka 224 Letn. 26 Jan. 2026 0000-00-00 Zaloznikova Objavljeno apc 2025-03-08 2025-12-23 2026-01-26 616.248-053.5 1471-2431 10.1186/s12887-025-06485-9 267940867 berce_association_2026.pdf berce_association_2026.pdf 1 4DABA3881830AE2F5D652D26867B36E3 c899203b9216810d28ef8211688e0be92fedbed5b47556daa15bc275782c8dd0 f4c277a8-738c-11f1-a785-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=46415 https://link.springer.com/article/10.1186/s12887-025-06485-9 1 e0a64bd1-7389-11f1-a785-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=46414 Nacionalni laboratorij za zdravje, okolje in hrano 0 0 0