20.500.12556/DiRROS-30466 Introduction of a spectrophotometric method for salivary iodine determination on microplate based on Sandell-Kolthoff reaction Uvedba spektrofotometrične metode za določanje joda v slini s pomočjo Snadell-Kolthoffove reakcije na mikrotitrski ploščici Background. Iodine is an essential element for the synthesis of thyroid hormones. Therefore, a reliable marker of iodine supply is important. Iodine is predominantly excreted via kidneys, but also via salivary glands. Our aim was to introduce a new and simple method for determination of salivary iodine concentration (SLIC). Materials and methods. Self-prepared chemicals and standards for Sandell-Kolthoff reaction on microplate with ammonium peroxydisulfate (AP) in the range 0−400 µg/L were used. Suitability of water-based standards (WBS) and artificial saliva-based standards (ASS) for standard curve were tested. We followed standards for method validation, defined concentration of used AP and compared our results with Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Results. WBS gave more reliable results than ASS as an underestimation of iodine concentration was found for ASS. LoB was 6.5 µg/L, LoD 12.0 µg/L, therefore analytical range was 12−400 µg/L. Intra- and inter-assay imprecisions at iodine concentrations, namely 20, 100, 165, and 350 µg/L were 18.4, 5.1, 5.7, and 2.8%, respectively, and 20.7, 6.7, 5.1, and 4.3%, respectively. Suitable molarity of AP was 1.0 mol/L and showed no difference to 1.5 mol/L (P values for samples with concentration 40, 100, and 150 µg/L, were 0.761, 0.085, and 0.275, respectively), whereas there was a significant change using 0.5 mol/L (P<0.001). Saliva samples could be diluted up to 1:8. There was no interference of thiocyanate and caffeine up to 193.5 mg/L. Our original method was comparable to ICP-MS. Spaerman coefficient was 0.989 (95% CI: 0.984−0.993). Conclusions. The new method for SLIC determination is in excellent agreement with ICP-MS and easy-to-use. iodine salivary iodine concentration Sandell-Kolthoff reaction true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik Neznano 2026-06-26 13:56:57 2026-06-26 13:56:57 2026-06-27 03:52:14 0000-00-00 00:00:00 2024 0 0 Soavtorji: Jernej Imperl, Mitja Kolar, Gregor Marolt, Blaz Krhin, Katja Zaletel, Simona Gaberscek; str. 357-365, V no. 3 Vol. 58 Sep. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-09-01 61 1318-2099 10.2478/raon-2024-0035 204612355 https://sciendo.com/article/10.2478/raon-2024-0035 1 24f7e9da-7156-11f1-b414-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=46268 RAZ_Oblak_Adrijana_2024.pdf RAZ_Oblak_Adrijana_2024.pdf 1 86A497BC695BC4403DF77544B9F36054 ccddf1c09cfa0a0a30eed32d7b5b48e19dbb46f68709ee8e166466f7f2f49502 541d6983-7156-11f1-b414-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=46269 Društvo radiologije in onkologije 0 0 0