20.500.12556/DiRROS-30457 Analysis of magnetic resonance contrast agent entrapment following reversible electroporation in vitro Analiza ujetja magnetnoresonančnega kontrastnega sredstva v celicah po reverzibilni elektroporaciji in vitro Background. Administering gadolinium-based contrast agent before electroporation allows the contrast agent to enter the cells and enables MRI assessment of reversibly electroporated regions. The aim of this study was evaluation of contrast agent entrapment in CHO cells and comparison of these results with those determined by standard in vitro methods for assessing cell membrane permeability, cell membrane integrity and cell survival following electroporation. Materials and methods. Cell membrane permeabilization and cell membrane integrity experiments were performed using YO-PRO-1 dye and propidium iodide, respectively. Cell survival experiments were performed by assessing metabolic activity of cells using MTS assay. The entrapment of gadolinium-based contrast agent gadobutrol inside the cells was evaluated using T1 relaxometry of cell suspensions 25 min and 24 h after electroporation and confirmed by inductively coupled plasma mass spectrometry. Results. Contrast agent was detected 25 min and 24 h after the delivery of electric pulses in cells that were reversibly electroporated. In addition, contrast agent was present in irreversibly electroporated cells 25 min after the delivery of electric pulses but was no longer detected in irreversibly electroporated cells after 24 h. Inductively coupled plasma mass spectrometry showed a proportional decrease in gadolinium content per cell with shortening of T1 relaxation time (R2 = 0.88 and p = 0.0191). Conclusions. Our results demonstrate that the contrast agent is entrapped in cells exposed to reversible electroporation but exits from cells exposed to irreversible electroporation within 24 h, thus confirming the hypothesis on which detection experiments in vivo were based. electroporation membrane permeabilization magnetic resonance contrast agent T1 relaxometry elektroporacija permeabilizacija membrane magnetnoresonančno kontrastno sredstvo T1 relaksometer true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik Neznano 2026-06-26 10:35:12 2026-06-26 10:35:12 2026-06-27 03:52:13 0000-00-00 00:00:00 2024 0 0 str. 406-415, X no. 3 Vol. 58 Sep. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-09-01 602.621:621.3 1318-2099 10.2478/raon-2024-0047 207898627 https://www.radioloncol.com/index.php/ro/article/view/4457 1 f3e74668-7139-11f1-b414-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=46253 RAZ_Strucic_Marko_2024.pdf RAZ_Strucic_Marko_2024.pdf 1 6F535393C979004EF316A1210C5425A6 f23eb2fbdb4d65f183383f22daa6e1424a004b3e0e69dd2526b9c179b8f2fa85 4e9f9fae-713a-11f1-b414-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=46254 Društvo radiologije in onkologije 0 0 0