20.500.12556/DiRROS-30210 Invasive properties of patient-derived glioblastoma cells after reversible electroporation in vitro Spremembe invazivnih lastnosti primarnih glioblastomskih celic po izpostavitvi reverzibilni elektroporaciji Background. Electroporation-based therapies are being explored in glioblastoma (GB) treatment, as means of enhancing drug delivery or achieving nonthermal ablation. Yet, little is known about how sublethal exposure affects the invasive behaviour of GB tumour cells. Materials and methods. Five patient-derived GB cell lines were initially screened for intrinsic invasive potential, and two most invasive (NIB140 CORE and NIB216 CORE) were selected for further experiments with electroporation treatment. Cells in suspension were exposed to bursts of high-frequency biphasic electric pulses resulting in electric field strength of 1 kV/cm, which corresponded to conditions of reversible electroporation. Changes in cell invasion and gene regulation were assessed 24 hours after electroporation using transwell assay and RNA transcriptome analysis, respectively. Results. Reversible electroporation at 1.0 kV/cm enhanced invasion in a cell line-dependent manner. NIB140 CORE showed a consistent and pronounced increase, with a median of 3.74-fold (274%) higher number of invading cells compared to sham control. In contrast, NIB216 CORE exhibited only a modest increase in invasion (1.30-fold; 30%). Transcriptomic profiling identified modulation of genes linked to extracellular matrix organization and ion channel activity in NIB140 CORE, and cytoskeletal remodelling in NIB216 CORE, indicating the activation of invasion-related pathways. Conclusions. These findings highlight a potential risk of pro-invasive responses in GB cells. In tumour ablation with irreversible electroporation, this concern relates to cells in the peripheral zone that may experience only sublethal electric fields, while in electrochemotherapy, a similar risk may arise if permeabilized cells are not effectively eliminated due to insufficient local drug delivery. Nevertheless, the two tested cell lines responded differently, underscoring patientspecific heterogeneity and the need for validation in more physiologically relevant models. electroporation high-frequency electric pulses glioblastoma patient-derived cells invasion elektroporacija visokofrekvenčni bipolarni pulzi glioblastom primarne celične kulture invazija true false true Association of Radiology and Oncology Radiology and oncology Angleški jezik Slovenski jezik by Authors Neznano 2026-06-17 13:57:13 2026-06-17 13:57:14 2026-06-18 04:07:45 0000-00-00 00:00:00 2025 0 0 str. 533-550, VIII no. 4 Vol. 59 Dec. 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2025-12-01 602.621 1318-2099 10.2478/raon-2025-0058 265177347 https://www.radioloncol.com/index.php/ro/article/view/4701 1 b021c0bd-6a43-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45765 https://reference-global.com/article/10.2478/raon-2025-0058 1 afbdaf3b-6a43-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45764 RAZ_Blazic_Anja_2025.pdf RAZ_Blazic_Anja_2025.pdf 1 5AD01C279C47D686AE8867D326385149 be55e63bcc4da55c6156d90a51d27c42d5ef96f3323e414ed03e49fc2fae5936 c8c683e7-6a43-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45769 Društvo radiologije in onkologije 0 0 0