20.500.12556/DiRROS-30208 Human papillomavirus (HPV) genotyping and prognostic value of HPV E4 protein and transcription factors NANOG and SOX11 in atypical p16 patchy squamous epithelium of cervix Genotipizacija človeških virusov papiloma (HPV) in napovedna vrednost beljakovine HPV E4 ter transkripcijskih faktorjev NANOG in SOX 11 v atipičnem ploščatem epitelu z lisasto izraženim p16 Background: Immunohistochemical staining for p16 is used to differentiate precancerous cervical lesions in tissue samples, but the interpretation of patchy p16 expression remains challenging. We performed human papillomavirus (HPV) genotyping and evaluated immunohistochemical expression of HPV E4 protein - a marker for transient infections, stem cell transcription factor NANOG, and transcription factor SOX11 to detect possible high-grade squamous lesions in atypical p16 patchy squamous epithelium. Materials and methods: We analyzed 24 cervical tissue samples with atypical squamous epithelium and patchy p16 expression along with the following controls: 11 cases of atypical squamous epithelium with null p16 expression, 9 condylomas, 12 cases of cervical intraepithelial neoplasia (CIN) grade 1, 11 cases of CIN2, and 9 cases of CIN3. In addition, HPV genotyping of tissue and related cervical smears from up to two years prior to biopsy was performed. Immunohistochemical staining for Ki67, HPV E4, NANOG, and SOX11 was performed and compared with follow-up data. Results: High-risk HPV infection was detected in 6/24 cases with patchy p16 expression and HPV E4 was expressed in 1/24 cases with patchy p16, weak NANOG expression was found in 11/24 cases with patchy p16 expression while no SOX11 expression was observed. During 10 months of follow-up, additional CIN1 and two CIN3 were identified, and another CIN1 and CIN3 after 5 and 6 years, accordingly. Conclusions: Our study showed that atypical squamous epithelium with patchy p16 expression poses a risk for highgrade precancerous lesions, harbouring high-risk HPV infection. Novel markers may hold diagnostic value in other specific contexts. NANOG SOX11 cervical intraepithelial neoplasia genotype human papillomavirus p16 cervikalna intraepitelna neoplazija genotip humani papiloma virus true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2026-06-17 13:53:28 2026-06-17 13:53:29 2026-06-18 04:07:44 0000-00-00 00:00:00 2025 0 0 Soavtorji: Anja Ostrbenk, Mario Poljak, Margareta Strojan-Flezar; str. 391-402, IX no. 3 Vol. 59 Sep. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-09-01 616:578.827 1318-2099 10.2478/raon-2025-0038 259531011 https://www.radioloncol.com/index.php/ro/article/view/4625 1 2a4833ff-6a43-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45762 RAZ_Kebe_Radulovic_Maja_2025.pdf RAZ_Kebe_Radulovic_Maja_2025.pdf 1 90160BF655AB46B45A5C5249BB4F414A 4e0a49181818438f277707a05c7af4e0097b999767c577aa2cef96bf656eca9e 47225793-6a43-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45763 Društvo radiologije in onkologije 0 0 0