20.500.12556/DiRROS-30207 Expression of the stem cell markers NANOG and SOX2 in the cervical squamous carcinogenesis Izražanje označevalcev matičnih celic NANOG in SOX2 v ploščatocelični karcinogenezi materničnega vratu Background: The aim of the present study was to assess a diagnostic potential of stem cell markers NANOG and SOX2 for classifying cervical squamous intraepithelial lesions (SILs)/cervical intraepithelial neoplasia (CIN). Patients and methods: NANOG and SOX2 expression was evaluated immunohistochemically on 40 patients: in 10 cases each of low-grade SIL (LSIL), high-grade SIL/CIN, grade 2 (HSIL/CIN 2), HSIL/CIN, grade 3 (HSIL/CIN 3), cervical squamous cell carcinoma (CSCC) and their adjacent non-dysplastic squamous epithelium. In addition, human papillomavirus (HPV) genotyping and immunohistochemical staining with p16 and Ki-67 were done. NANOG and SOX2 expression was compared between squamous lesions and controls and between squamous lesions by multiplying staining intensity (SI) by the percentage of positive cells (P) and by multiplying SI by the thickness of staining in epithelium (T) to calculate SI x P and SI x T score. Results: NANOG and SOX2 expression gradually increased from non-dysplastic squamous epithelium via LSIL and HSIL to CSCC. Expression of NANOG and SOX2 was higher in LSIL compared to controls (P < 0.05 for NANOG Si x P and Si x T scores and SOX2 SI x T score) and lower compared to HSIL (P < 0.05 for all SI x P and SI x T scores). HSIL/CIN 3 showed higher SOX2 expression than HSIL/CIN 2 (P < 0.05 for SI x P and SI x T scores). Conclusions: Contrary to p16, NANOG and SOX2 could be effective for distinguishing LSIL from non-dysplastic changes. NANOG and SOX2 could be surrogate markers for differentiating LSIL from HSIL. Moreover, SOX2 could be helpful for distinguishing HSIL/CIN 2 from HSIL/CIN 3. Further studies with larger numbers of patients and molecular insights are needed. cervical cancer cervical intraepithelial neoplasia squamous intraepithelial lesion cancer stem cell NANOG SOX2 rak materničnega vratu intraepitelijska neoplazija materničnega vratu skvamozan intraepitelijska lezija matične celice raka true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2026-06-17 13:44:00 2026-06-17 13:44:00 2026-06-18 04:07:44 0000-00-00 00:00:00 2025 0 0 Soavtorji: Margareta Zlajpah, Mario Poljak, Kristina Fujs Komlos, Margareta Strojan Flezar; str. 213-224, VI no. 2 Vol. 59 Jun. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-06-01 616-0006 1318-2099 10.2478/raon-2025-0026 235704067 RAZ_Koren_Miha_2025.pdf RAZ_Koren_Miha_2025.pdf 1 3C10CCF559AF47D722BFB228ADAE0C5B f94d96043acdabefc86c9fe6786a01471e6b3cadd7ca8042be64f959486b08b8 f49c8488-6a41-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45759 https://sciendo.com/article/10.2478/raon-2025-0026 1 d6c71e9b-6a41-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45758 Društvo radiologije in onkologije 0 0 0