20.500.12556/DiRROS-30185 Associations of PFAS and phthalate/DINCH metabolites with metabolic regulation in teenagers from the HBM4EU aligned studies Background. Exposure to per- and polyfluoroalkyl substances (PFAS) and phthalates is widespread during adolescence, a critical developmental period for metabolic regulation. Aim. To assess associations of serum PFAS and urinary phthalate/DINCH metabolites-individually and as mixtures-with a unified panel of metabolic biomarkers representing adipose-brain-liver cross-talk and oxidative stress in European teenagers. Methodology. Serum PFAS and urinary phthalate/DINCH metabolites were measured in 1033 European teenagers (12–17 years) from the Human Biomonitoring Initiative for Europe (HBM4EU) Aligned Studies. Metabolic biomarkers representing adipose (HDL, LDL, cholesterol, and triglycerides)-brain (leptin, adiponectin, and kisspeptin)–liver (glucose, insulin) cross-talk and oxidative stress (8-hydroxy-2′-deoxyguanosine, 8OHdG) were measured. The Body Mass Index z-score (zBMI) was calculated. Single pollutant models, multivariate MANOVA, quantile g-computation, and BKMR models were fit, including interaction terms with sex. Results. Single pollutant models showed positive associations of PFAS and phthalate/DINCH metabolites with 8OHdG. PFAS were associated with higher leptin, HDL, LDL, and cholesterol, while some phthalate/DINCH metabolites were associated with lower kisspeptin, HDL, triglycerides, cholesterol, zBMI, and higher adiponectin. We observed weak but statistically significant associations between PFAS and phthalate/DINCH metabolites with the entire set of metabolic biomarkers in the MANOVA. The PFAS mixture was associated with higher kisspeptin, LDL, HDL, cholesterol, and 8OHdG. The phthalate/DINCH mixture was associated with lower HDL. Conclusions. Exposure to these contaminants may be related to dyslipidemia in teenagers. PFAS and phthalate/DINCH metabolites may exert opposite associations on metabolism, with the exception of increasing oxidative stress. Given the cross‑sectional design and potential residual confounding, longitudinal studies are warranted. human biomonitoring DINCH metabolites HBM4EU metabolic regulation perfluoroalkyl substances polyfluoroalkyl substances metaboliti HBM4EU regulacija metabolizma perfluorirane alkilne spojine polifluorirane alkilne spojine true false true Elsevier Nizozemska Angleški jezik Slovenski jezik Evropska unija; © 2026 The Author(s). Neznano 2026-06-16 15:19:18 2026-06-16 15:19:18 2026-06-17 03:46:53 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorja iz Slovenije: Tina Kosjek, Žiga Tkalec; Opis vira z dne 15. 5. 2026; str. [1-36] Vol. , article no. 110302 [in press] 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2026-01-16 2026-05-12 2026-05-18 504.5 1873-6750 10.1016/j.envint.2026.110302 278295043 https://www.sciencedirect.com/science/article/pii/S0160412026002606 1 fd22b1b4-6985-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45698 RAZ_Rodriguez-Carillo_Andrea_2026.pdf RAZ_Rodriguez-Carillo_Andrea_2026.pdf 1 37B731836B20EB08230686B1189493CE 3b542da7979ea5fb6fe43e8c3a1d27fc1d31ea441c1b42c14677a23e34431d8b 5481b450-6986-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45699 Institut Jožef Stefan 0 0 0