20.500.12556/DiRROS-30161 An LC-MS/MS method for quantification of lamotrigine and its main metabolite in dried blood spots Background: The antiepileptic drug lamotrigine (LTG) shows high pharmacokinetic variability due to genotype influence and concomitant use of glucuronidation inducers and inhibitors, both of which may be frequently taken by elderly patients. Our goal was to develop a reliable quantification method for lamotrigine and its main glucuronide metabolite lamotrigine-N2-glucuronide (LTG-N2-GLU) in dried blood spots (DBS) to enable routine therapeutic drug monitoring and to identify altered metabolic activity for early detection of drug interactions possibly leading to suboptimal drug response. Results: The analytical method was validated in terms of selectivity, accuracy, precision, matrix effects, haematocrit, blood spot volume influence, and stability. It was applied to a clinical study, and the DBS results were compared to the concentrations determined in plasma samples. A good correlation was established for both analytes in DBS and plasma samples, taking into account the haematocrit and blood cell-to-plasma partition coefficients. It was demonstrated that the method is suitable for the determination of the metabolite-to-parent ratio to reveal the metabolic status of individual patients. Conclusions: The clinical validation performed confirmed that the DBS technique is a reliable alternative for plasma lamotrigine and its glucuronide determination. dried blood spot lamotrigine lamotrigine glucuronide therapeutic drug monitoring clinical validation haematocrit effect posušena krvna lisa lamotrigin lamotrigin glukuronid terapevtsko spremljanje zdravil klinična validacija učinek hematokrita true false false Angleški jezik Slovenski jezik Neznano 2026-06-16 11:44:28 2026-06-16 11:44:28 2026-06-17 03:46:51 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 4. 4. 2024; 16 str. no. 4, [Article no.] 449 Vol. 17 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.015:616.853 1424-8247 10.3390/ph17040449 191424771 RAZ_Milosheska_Daniela_2024.pdf RAZ_Milosheska_Daniela_2024.pdf 1 CA6DA12A4BC76D6FD2F8E3C8F133899F 327c819cb2d761f253b455fa21772218e836c02557e1d70ea61aa4369878fc8d 2a1f1ab7-6968-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45633 https://www.mdpi.com/1424-8247/17/4/449 1 f9306d69-6967-11f1-97b2-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45632 Univerzitetni klinični center Ljubljana 0 0 0