20.500.12556/DiRROS-29994 Molecular maps of synovial cells in inflammatory arthritis using an optimized synovium dissociation protocol In this study, we optimized the dissociation of synovial tissue biopsies for single-cell omics studies and created a single-cell atlas of human synovium in inflammatory arthritis. The optimized protocol allowed consistent isolation of highly viable cells from tiny fresh synovial biopsies, minimizing the synovial biopsy drop-out rate. The synovium scRNA-seq atlas contained over 100000 unsorted synovial cells from 25 synovial tissues affected by inflammatory arthritis, including 16 structural, 11 lymphoid and 15 myeloid cell clusters. This synovial cell map expanded the diversity of synovial cell types/states, detected synovial neutrophils, and broadened synovial endothelial cell classification. We revealed tissue-resident macrophage subsets with proposed matrix-sensing (FOLR2+COLEC12high) and iron-recycling (LYVE1+SLC40A1+) activities and identified fibroblast subsets with proposed functions in cartilage breakdown (SOD2highSAA1+SAA2+SDC4+) and extracellular matrix remodeling (SERPINE1+COL5A3+LOXL2+). Our study offers an efficient synovium dissociation method and a reference scRNA-seq resource, that advances the current understanding of synovial cell heterogeneity in inflammatory arthritis. molecular maps synovial cells inflammatory arthritis synovium dissociation protocol molekularne karte sinovialne celice vnetni artritis protokol za disociacijo sinoviuma true false true Angleški jezik Slovenski jezik Neznano 2026-06-11 09:56:33 2026-06-11 09:56:34 2026-06-12 03:54:41 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 9. 5. 2024; str. 1-28 iss. 6, [article no.] 109707 Vol. 27 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-002 2589-0042 10.1016/j.isci.2024.109707 195037699 RAZ_Edalat_Sam_G._2024.pdf RAZ_Edalat_Sam_G._2024.pdf 1 C5E30C524387E829101D65BF619458FC f202ec7c5ccf3807e83c675643e5999ec377acfda55243583fd35642d1daa22d 3a37c287-656b-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45194 https://www.cell.com/action/showPdf?pii=S2589-0042%2824%2900929-5 1 124fd4be-656b-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45193 Univerzitetni klinični center Ljubljana 0 0 0