20.500.12556/DiRROS-29950 Optimised extraction and HPLC–DAD determination of steroidal glycoalkaloids in potato tubers analytical validation and environmental sustainability evaluation Steroidal glycoalkaloids ( α-solanine and α-chaconine) are major potato toxins whose accurate quantification is often challenged by matrix effects and chromatographic selectivity. This study reports the optimisation and validation of a robust reversed-phase HPLC-DAD method for the determination of glycoalkaloids in potato tissues, combined with a systematic assessment of the environmental sustainability of the analytical workflow. Extraction conditions were optimized by integrating systematic solvent screening with a factorial experimental design to evaluate the effect of key operational variables on glycoalkaloid recovery and refine extraction performance The resulting extraction medium improved extract cleanliness and chromatographic stability. A subsequent C18 solid-phase extraction SPE step further reduced matrix effect and enhanced analytical selectivity. Chromatographic separation was achieved on a conventional C18 column using an acetonitrile-water gradient, providing baseline resolution (Rs > 1.5) of α 2 -solanine and α-chaconine with stable diode-array detection (DAD) at 205 nm. The method was validated according to ICH Q2(R2) and AOAC guidelines, demonstrating excellent linearity (R > 0.995), low limits of detection (0.462–0.767 µg/mL) and quantification (1.540–2.558 µg/mL), high precision (≤ 5% RSD), and recoveries of 90–105%. Robustness testing confirmed minimal sensitivity to routine variations in chromatographic parameters. Application to potato breeding lines demonstrated reliable quantification and revealed genotype-dependent variation in glycoalkaloid content. The environmental assessment of the analytical procedure was evaluated using Analytical Eco-scale, GAPI and AGREE metrics, demonstrating reduced solvent consumption, avoidance of strongly acidic extraction conditions, and a simplified sample preparation workflow. The proposed HPLC-DAD workflow provides a validated, high-performance, and environmentally conscious approach for routine GA quantification, suitable for food-safety monitoring, regulatory compliance and breeding programme applications. α-chaconine α-solanine analytical method HPLC-DAD potato tubers environmental sustainability Solanum tuberosum true false true Angleški jezik Latinski jezik Neznano 2026-06-10 09:12:36 2026-06-10 09:12:36 2026-06-11 03:47:34 0000-00-00 00:00:00 2026 0 0 Soavtorji: Peter Dolničar, Barbara Pipan, Lovro Sinkovič; str. 1-18 Vol. 1783, [article no.] 467165 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-06-03 633 1873-3778 10.1016/j.chroma.2026.467165 280684547 https://www.sciencedirect.com/science/article/pii/S0021967326004942?via%3Dihub 1 c35f427c-649b-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45106 RAZ_Ben_Ammar_Hajer_2026.pdf RAZ_Ben_Ammar_Hajer_2026.pdf 1 3DD23369587B8174FBA374C4E3AD50C6 b741dcae51b5d677ed0073e55f25bcf75367a07bf58657038255cc4c0c72d04e dd9432de-649b-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45107 Kmetijski inštitut Slovenije 0 0 0