20.500.12556/DiRROS-29937 Analysis-associated factors interfering with diffusion tensor indices of peripheral nerves Z analizo povezani dejavniki, ki vplivajo na značilke difuzijskih tenzorjev perifernih živcev Diffusion tensor imaging (DTI) enables a non-invasive assessment of tissue architecture based on water diffusion. Advancements in technology have expanded the application of DTI from the central nervous system to the peripheral nervous system. High-resolution magnetic resonance imaging (MRI) systems have further enhanced the visualization of nerve compartments. This study investigates the influence of region of interest (ROI) selection on DTI indices in ex vivoperipheral nerves. Cadaveric median nerves (n=10) were harvested and immersed in fluorinated-carbon liquid, and 9-mm segments were independently scanned with a 9.4-T MRI system (scanning time 40 h). Diffusion-weighted signals were summed, and diffusion tensors were calculated for anatomical compartments using different delineation techniques. Following the initial scan, five samples were scanned for the second time using an identical protocol. The analysis showed that the inclusion of an inert background into the ROI significantly reduces fractional anisotropy (FA). Significant differences in diffusion tensors were observed between the initial and intermediate nerve portions, with FA and mean diffusivity (MD) differing by as much as 25.2% and 25.6%, respectively. Tracing the fascicles without the interfascicular epineurium exhibited an 8.8% lower FA compared to the delineations of the epineurium. Second MRI scans showed significant changes in diffusion tensors with higher eigenvalues D1-D3 and MD. In conclusion, the intermediate portions of the nerve demonstrated greater consistency in DTI indices and are therefore recommended for analysis over the initial portions. Using an inert liquid can minimize background effects, but the inherent diffusion properties of the tissue must be carefully considered. Prolonged scanning times can alter diffusion tensors, likely due to autolytic processes, underscoring the importance of consistent pre-scanning conditions and acquisition protocols. Additionally, maintaining consistency in delineations is paramount, regardless of whether the tracing method includes or excludes the interfascicular epineurium. diffusion tensor imaging fractional anisotropy image analysis perineural tissue peripheral nerve difuzijsko tenzorsko slikanje frakcijska anizotropija analiza slike perinevralno tkivo periferni živec true false true Angleški jezik Slovenski jezik Neznano 2026-06-09 13:57:54 2026-06-09 13:57:55 2026-06-10 03:48:59 0000-00-00 00:00:00 2024 0 0 Povzetek v slov.: Z analizo povezani dejavniki, ki vplivajo na značilke difuzijskih tenzorjev perifernih živcev; str. 203-210 no. 3 Vol. 43 Nov. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-07 1580-3139 10.5566/ias.3332 214139907 RAZ_Pusnik_Luka_2024.pdf RAZ_Pusnik_Luka_2024.pdf 1 9A5F1B3C7C406E43B48A6E1AB5683398 49fd8eb946eaf2f056b029f2f94ae893aff6f7479ddd3426466355fcb114e54e 92af6c25-63fa-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45086 https://www.ias-iss.org/ojs/IAS/article/view/3332/1220 1 755b795f-63fa-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=45085 Univerzitetni klinični center Ljubljana 0 0 0