20.500.12556/DiRROS-29804 Associations between APOE genotypes, urine 8-isoprostane and blood trace elements in middle-aged mothers (CROME study) Background. There is almost no data on the combined associations between apolipoprotein E gene (APOE) genotypes, trace elements (TEs), and lipid peroxidation in vivo. The aim of our study was to evaluate the association between APOE genotypes and TE levels in blood (B-TEs) and erythrocytes (E-TEs), and 8-isoprostane in urine (U-8-isoprostane) in women with low exposure to potentially toxic TEs and with adequate supply of essential TEs. Methods. B-TEs, E-TEs and U-8-isoprostane were determined in 172 healthy women of childbearing age (30.1–51.4 years) using ICP-MS and ELISA competitive assay, respectively. All women were divided into three APOE genotype groups according to the presence of the ɛ4 allele, ɛ2 allele or ɛ3 homozygotic allele. The associations between B-TEs, E-TE, U-8-isoprostane, and the APOE genotype groups were estimated by multiple variable linear regression models with relevant explanatory variables (e.g., age, BMI, and seafood). Results. All TE and U-8-isoprostane levels were inside the reference ranges for the healthy population. In the multiple variable linear regression models, our results showed that urine 8-isoprostane levels increased by up to 43.3% in the APOE4 group compared to the APOE3 group and a negligible negative modifying effect for essential TEs. However, the APOE genotype groups were associated also with some TEs. A clear positive association was found between the APOE2 and APOE4 groups (vs. APOE3) with B-molybdenum. Conclusions. Our study suggests that the APOE4 genotype played an important role in 8-isoprostane variability in a population with an adequate supply of essential and with low exposure to potentially toxic TEs. Adequate copper, zinc and selenium status seemed to be protective against, while the levels of nonessential TEs were probably too low to play a decisive role in 8-isoprostane formation. The observed impact of the APOE2 and APOE4 groups on increased B-molybdenum opens a new research topic. apolipoprotein E polymorphisms trace elements multiple variable linear regression models apolipoprotein E polimorfizmi sledovi kemijskih elementov true false true Angleški jezik Slovenski jezik Neznano 2026-06-05 13:34:26 2026-06-05 13:34:27 2026-06-06 03:58:35 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 24. 10. 2024; str. 1-14 Vol. 193, [article no.] 109034 Nov. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 504.5 1873-6750 10.1016/j.envint.2024.109034 212805891 RAZ_France_Stiglic_Alenka_2024.pdf RAZ_France_Stiglic_Alenka_2024.pdf 1 F6FC8992EC61A5BA8A89B5C6285D9423 c42478794e7329d22fd7c370ee22089043f7d53469b09aed147fcfeefb271200 b594db00-60d2-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44809 https://www.sciencedirect.com/science/article/pii/S0160412024006202 1 8469dbdd-60d2-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44808 Univerzitetni klinični center Ljubljana 0 0 0