20.500.12556/DiRROS-29722 The influence of late gadolinium enhancement cardiac magnetic resonance image analysis imprecision on myocardial damage quantification in patients with myocarditis a pilot study Background: Myocardial damage in myocarditis is assessed through late gadolinium enhancement (LGE) cardiac magnetic resonance (CMR). Variability in quantifying myocarditis extent results from imprecise image segmentation and inconclusive data on quantification method selection. To improve analysis precision, segmentation steps are systematically ranked based on their inherent risks of error. Additionally, data on two distinct quantification methods are presented. Methods: Using newly developed software, four experts analyzed five LGE-CMR left ventricular (LV) short-axis (SAx) images of myocarditis patients in three sessions. Regions of interest (ROIs) (myocardial (ROImyoc), reference (ROIref), and exclusion region (ROIexcl)) were identified and used to calculate LGE extent with 3σ (intensity above three standard deviations (σ) in reference) and the full width at half maximum (FWHM) method (intensity above 50% of maximum signal in reference). The reference LGE extent was calculated and the influence of the ROIs on LGE extent variability was determined. Interobserver and intraobserver variability were evaluated as 1-intraclass correlation coefficient (ICC). Results: LGE extent variability was 6.2 ± 0.6% for 3σ and 4.0 ± 0.6% for FWHM. The contributions of ROImyoc, ROIref, and ROIexcl were 1.5 ± 0.2%, 2.7 ± 0.4%, and 2 ± 0.3%, respectively, for 3σ, and 1.1 ± 0.1%, 1.6 ± 0.4%, and 1.3 ± 0.3%, respectively, for FWHM. LGE extent was lower in FWHM. Interobserver variability was 0.56 for 3σ and 0.43 for FWHM. The intraobserver variability was higher for the 3σ method in all four observers. Conclusion: ROIref selection contributed most to LGE extent variability. FWHM yielded lower LGE extent and lower inter- and intraobserver variability. Due to low statistical significance, the findings are only partially confirmed. myocarditis cardiac magnetic resonance variability full width at half maximum thresholding methods segmentation miokarditis magnetna resonanca srca variabilnost polna širina na polovici maksimuma metode določanja pragov segmentacija true false true Angleški jezik Slovenski jezik Neznano 2026-06-03 14:58:13 2026-06-03 14:58:14 2026-06-04 04:04:46 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 15. 3. 2024; str. 1-11 iss. 1, [article no.] 117 Vol. 14 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.1:612 2076-3417 10.3390/app14010117 189025795 RAZ_Kralj_Lana_2024.pdf RAZ_Kralj_Lana_2024.pdf 1 82C45017F8D5FDF7B21DD5809BC313B3 34a00ff7f24564afd573a0306411da4c3b0288e2b423b270685a8ab5c030e754 0034fa12-5f4c-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44659 https://www.mdpi.com/2076-3417/14/1/117 1 e2e69a25-5f4b-11f1-9215-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44658 Univerzitetni klinični center Ljubljana 0 0 0