20.500.12556/DiRROS-29646 Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation Objectives: Peptidoglycan (PG) is an arthritogenic bacterial cell wall component whose role in human osteoarthritis is poorly understood. The purpose of this study was to determine if PG is present in synovial tissue of osteoarthritis patients at the time of primary total knee arthroplasty (TKA), and if its presence is associated with inflammation and patient reported outcomes. Methods: Intraoperative synovial tissue and synovial fluid samples were obtained from 56 patients undergoing primary TKA, none of whom had history of infection. PG in synovial tissue was detected by immunohistochemistry (IHC) and immunofluorescence microscopy (IFM). Synovial tissue inflammation and fibrosis were assessed by histopathology and synovial fluid cytokine quantification. Primary human fibroblasts isolated from arthritis synovial tissue were stimulated with PG to determine inflammatory cytokine response. Results: A total of 33/56 (59%) of primary TKA synovial tissue samples were positive for PG by IHC, and PG staining colocalized with markers of synovial macrophages and fibroblasts by IFM. Synovial tissue inflammation and elevated IL-6 in synovial fluid positively correlated with PG positivity. Primary human fibroblasts stimulated with PG secreted high levels of IL-6, consistent with ex vivo findings. Interestingly, we observed a significant inverse correlation between PG and age at time of TKA, indicating younger age at time of TKA was associated with higher PG levels. Conclusion: Peptidoglycan is commonly found in synovial tissue from patients undergoing TKA. Our data indicate that PG may play an important role in inflammatory synovitis, particularly in patients who undergo TKA at a relatively younger age. osteoarthritis peptidoglycan synovitis inflammation true false true Angleški jezik Ni določen Neznano 2026-06-02 09:58:59 2026-06-02 09:59:00 2026-06-03 03:56:37 0000-00-00 00:00:00 2024 0 0 Nasl z nasl. zaslona; Opis vira z dne 25. 5. 2026; str. 1-11 iss. 1, [article no.] 77 Vol. 26 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.7 1478-6362 10.1186/s13075-024-03293-x 279280131 RAZ_Holub_Meaghan_N_2024.pdf RAZ_Holub_Meaghan_N_2024.pdf 1 CCB041303357FC9358E8DF54D75ACB40 90c9b15e4f326b2bf6738dedf5ff6eb92314f442da4f81a8059726e5f7594cb8 083db9b5-5e59-11f1-8e51-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44519 https://link.springer.com/article/10.1186/s13075-024-03293-x 1 ebe441ed-5e58-11f1-8e51-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44517 Univerzitetni klinični center Ljubljana 0 0 0