20.500.12556/DiRROS-29600 Adipose tissue transcriptome profiles of local Krškopolje pig and modern hybrids receiving reduced protein diets Compared to lean pig genotypes, the local Krškopolje pig is characterized by a greater capacity for lipid and lower capacity for protein deposition, along with lower dietary protein requirements. The aim of the present study was to unravel the transcriptomic differences in subcutaneous adipose tissue between the local Krškopolje pig and modern hybrids, as well as to study the impact of the reduced protein diet in both genotypes. Differential gene expression analysis between local and modern pigs revealed 375 differentially expressed genes, with 189 upregulated and 186 downregulated in Krškopolje pig. Among the upregulated genes, several suggested more pronounced adipogenesis (SLC7A10, ADIRF, INHBB, and SFRP2) in the Krškopolje pig. In addition, genes encoding collagen proteins and extracellular matrix remodeling (e.g., COL6A5, COL4A5, COL2A1), calcium signalling (TRPV4, CAMK2A, CALML5), pro-inflammatory cytokines (e.g., IL1A, TNFSF9, CXCL8, PTGS2 etc.) and cholesterol metabolism (CYP1A1, CYP2A19, CES1 etc.) were differentially expressed. The reduced protein diet induced only minor changes in gene expression at the individual gene level. However, gene set enrichment analysis revealed that, in Krškopolje pigs, dietary effects were associated with biological processes related to immune response, intracellular protein transport, and extracellular matrix organization. In the case of modern hybrids, reduced protein diet resulted in enrichment of gene sets related to oxidative phosphorylation, mitochondrial function, and energy metabolism. In conclusion, this study demonstrated that the adipose tissue of Krškopolje pigs compared with modern hybrids exhibited greater adipogenic potential, being also supported by higher expression of genes involved in extracellular matrix remodelling and inflammation. The impact of reduced protein diet on adipose-tissue gene expression was relatively small in both breeds, particularly in Krškopolje pig, suggesting a metabolic adaptability of this breed. gene expression breed comparison low protein dietadipogenesisimmune response adipogenesis immune response true false false Angleški jezik Ni določen Neznano 2026-06-01 08:58:17 2026-06-01 08:58:17 2026-06-02 03:48:22 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: M. Čandek-Potokar, B. Savić, M. Škrlep; Opis vira z dne 1. 6. 2026; str. iss. 2, [articlo no.] 101864 Vol. 18 May 2026 0000-00-00 PreprintDelovna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-05-27 636.4 1751-732X 10.1016/j.animal.2026.101864 279939843 https://www.sciencedirect.com/science/article/pii/S1751731126001114 1 45dc43c8-5d87-11f1-8e51-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44421 RAZ_Poklukar_Znidarsic_Klavdija_2026.pdf RAZ_Poklukar_Znidarsic_Klavdija_2026.pdf 1 D55F58ED2AB9FE3846278F6D94267415 519c4ca24136e0d6fa789e46edd3ed5a25069b3789256e31d3c35a756ed83d4c 754607ba-5d87-11f1-8e51-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44422 Kmetijski inštitut Slovenije 0 0 0