20.500.12556/DiRROS-29485 Computational modeling and characterization methods for rotating magnetic nanochain-enhanced lateral flow immunoassays This article provides comprehensive methodological guidance for implementing rotating magnetic nanochain-enhanced lateral flow immunoassays with volumetric magnetic detection. Rotating magnetic nanochains act as microscale stirrers that substantially enhance antibody-antigen binding kinetics through convective mixing, yet their integration into lateral flow platforms presents unique technical challenges requiring both computational optimization and specialized characterization. We describe complete workflows for: (i) computational fluid dynamics modeling using COMSOL Multiphysics to simulate nanochain rotation, fluid flow, and mass transport enhancement; (ii) electron microscopy characterization of magnetic nanochain morphology and size distributions; (iii) rotating magnetic field generator design and operation; and (iv) magnetic particle quantification measurement procedures for volumetric signal readout. Each section provides step-by-step instructions with sufficient detail to enable independent replication. The described methods enable development of lateral flow assays achieving sub-nanogram detection limits with rapid (6-minute) analysis times, addressing critical needs in point-of-care diagnostics. These methods complement our related research article in Biosensors and Bioelectronics by providing the technical foundation necessary for adoption and adaptation of this technology by other laboratories. magnetic nanochains lateral flow immunoassay computational fluid dynamics point-of-care diagnostic rotating magnetic field cardiac biomarkers magnetne nanoverige diagnostika ob bolniku kardiološki biomarkerji true false true Elsevier Nizozemska Angleški jezik Slovenski jezik © 2026 The Authors. Neznano 2026-05-20 14:55:53 2026-05-20 14:55:53 2026-05-21 03:54:14 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtor iz Slovenije: Slavko Kralj; Opis vira z dne 19. 5. 2026; str. 1-18 Vol. 16, [article no.] 103936 Jun. 2026 0000-00-00 Zaloznikova Objavljeno apc 2026-03-12 2026-05-02 2026-05-05 620.3 2215-0161 10.1016/j.mex.2026.103936 278650115 https://www.sciencedirect.com/science/article/pii/S2215016126001548?via%3Dihub 1 3e3710d0-544b-11f1-8a5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44230 RAZ_Orlov_Alexey_V._2026.pdf RAZ_Orlov_Alexey_V._2026.pdf 1 EB89A116154563A042131FF24683C16D 665badad7fc35f76b72c24d1a33ae8d289979378bedbb0ddc32e568ea2c3394a a7e7c7a0-544b-11f1-8a5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44233 Institut Jožef Stefan 0 0 0