20.500.12556/DiRROS-29416 Gut microbiomes of wild and domesticated mammals and birds in Slovenia, Europe 16S rRNA sequencing data From a One Health perspective, the gut microbiota of animals acts as a major driver of microbial exchange between animals and the environment. Animals continuously release gut microbes into their surroundings, shaping environmental and human microbial communities and potentially dispersing pathogens. Characterizing gut microbiota across diverse animal hosts is therefore critical for understanding the patterns of microbial spread through ecosystems and their impact on animal, human and environmental health. Here, we introduce a large, taxonomically diverse dataset of fecal microbiomes from 715 individual animals representing over 50 mammalian and avian species. We collected samples from both wild and domestic animals with an emphasis on capturing microbial diversity across a wide range of taxa and ecological contexts. The samples were subjected to 16S rRNA gene sequencing, targeting the V3–V4 hypervariable region. Bioinformatic analysis was performed using Usearch to generate zero-radius operational taxonomic units (ZOTUs). This dataset was generated primarily for the development of microbial source tracking (MST) assays used for identifying the sources of fecal pollution in contaminated water. However, it provides a valuable resource for broader microbiome research. It enables comparative studies across host species, trophic guilds, and environmental contexts such as domestication. gut microbiota 16S rRNA gene metagenome animal feces črevesna mikrobiota gen 16S rRNA metagenom živalski iztrebki true false true Elsevier Angleški jezik Slovenski jezik Neznano 2026-05-18 11:46:13 2026-05-18 11:46:14 2026-05-19 03:54:39 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; opis vira z dne 15. 5. 2026 Str. 1-12 Letn. 65, [št. članka] 112146 Feb. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-11-26 2026-02-02 2026-02-09 579 2352-3409 10.1016/j.dib.2026.112564 278227203 zlender_rupnik_gut_2026.pdf zlender_rupnik_gut_2026.pdf 1 FAFBD7C8C40E95863814AF34C261AF2F f2192ebf11d9cc611fd6384181a0dc50664bae50dc096a05704b09b69d78f4fd 92a02d08-52a1-11f1-8a5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44104 https://www.sciencedirect.com/science/article/pii/S2352340925008674 1 69ce1849-529e-11f1-8a5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=44098 Nacionalni laboratorij za zdravje, okolje in hrano 0 0 0