20.500.12556/DiRROS-29274 Proximity-induced transfer of a mass tag enables direct profiling of active matrix metalloproteases Conventional activity-based probes in activity-based protein profiling (ABPP) require enrichment or reporter tags for detection, which limits sensitivity and multiplexing. Here, we present an enrichment-free chemoproteomic approach that enables direct mass spectrometric detection by Matrix-Assisted Laser Desorption/Ionization (MALDI) of active proteases. An active-site–directed affinity probe transfers, through a proximity-induced reaction, a MALDI-detectable α-cyano-4-hydroxycinnamic acid (CHCA) tag exclusively to catalytically active forms of matrix metalloproteases (MMPs). The CHCA label enhances ionization efficiency and markedly improves signal-to-noise ratios, allowing confident identification of CHCA-labelled peptides under discriminating analytical conditions. Each active metalloprotease is thereby, associated with a distinct set of CHCA signature peptides, defining its activity fingerprint. This workflow achieves multiplexed and quantitative activity profiling of MMPs, directly in complex proteomes. This design expands ABPP into the mass spectrometry domain and establishes a robust platform for activity-based enzyme detection. activity-based probes chemoproteomic mass tag proximity-induced labelin metaloproteaze rak (bolezen) inhibitorji true false true Angleški jezik Slovenski jezik Neznano 2026-05-05 15:30:29 2026-05-05 15:30:29 2026-05-06 03:51:49 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: Ugo Pasco, Mylene Sejalon-Cipolla, Pierrick Bruyat, Monika Kaminska, Carole Malgorn, Fabrice Beau, Manca Osolin, Metka Novak, Barbara Breznik, Dimitris Georgiadis, Isabelle Correia, Emeline Groult, Olivier Lequin, Sarah Bregant, Yves-Marie Pers, Daniele Noel, Robert Thai, Gilles Subra, Sonia Cantel, Laurent Devel; Opis vira z dne 17. 4. 2026; str. 1-11 iss. , [article no.] e23132 Vol. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-04-04 577.2 1521-3773 10.1002/anie.202523132 275650307 https://onlinelibrary.wiley.com/doi/10.1002/anie.202523132 1 973c5fb9-4886-11f1-8a5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43811 RAZ_Berthy_Lomane_2026.pdf RAZ_Berthy_Lomane_2026.pdf 1 C8AFE0C5DD7FCC80A86AE23972FF4EBB ed4b630f35d5141ad6a50fa2a16a1822e1cea9b65e760847cd6be880b8dcd3e7 aee4095b-4886-11f1-8a5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43812 Nacionalni inštitut za biologijo 0 0 0