20.500.12556/DiRROS-29223 Influence of particle size, shape, and magnetic properties on torque-driven biofilm removal using anisotropic magnetic particles Biofilms are structured communities of bacteria embedded within an extracellular polymeric substance (EPS) matrix, which forms a protective barrier that restricts drug penetration and increases antibiotic tolerance, making their complete elimination particularly challenging. Here, we investigate a magnetomechanical approach using rotating magnetic fields (RMFs) to deliver controlled mechanical stress to Enterococcus faecalis biofilms via anisotropic magnetic particles (AMPs). Microrods, nanochains, and nanorods with distinct sizes and magnetic properties were actuated under identical RMF conditions on implant-relevant titanium substrates. Micron-scale magnetic microrods generate sufficient magnetic torque to mechanically disrupt the EPS matrix and detach biofilm structures, significantly increasing suspended bacterial cells without marked bactericidal effects. In contrast, nanoscale AMPs do not induce biofilm detachment but cause membrane damage, increasing the proportion of injured cells. These findings demonstrate a size-dependent transition between microscale biofilm detachment and nanoscale membrane interactions, identifying particle size as the dominant parameter governing magneto-mechanical biofilm disruption. magnetomechanical biofilm disruption anisotropic magnetic particles nano–micro scale bacterial interactions magnetomehanska disruptcija biofilmov anizotropni magnetni delci interakcije nano- in mikromaterialov z bakterijami true false true The Royal Society of Chemistry Združeno kraljestvo Angleški jezik Slovenski jezik This journal is © The Royal Society of Chemistry 2026 Neznano 2026-04-28 10:15:14 2026-04-28 10:15:15 2026-04-29 03:43:58 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: Laurine Marger, Maja Caf, Fabrice Marger, Mustapha Mekki, Slavko Kralj, Irena Milošević; Opis vira z dne 23. 4. 2026; str. [1-12] iss. Vol. Mar. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2026-01-05 2026-03-26 2026-03-27 620.3 2040-3372 10.1039/D6NR00046K 276227075 https://pubs.rsc.org/en/Content/ArticleLanding/2026/NR/D6NR00046K 1 6e6a985a-42da-11f1-a4de-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43712 RAZ_Pautu_Vincent_2026.pdf RAZ_Pautu_Vincent_2026.pdf 1 1EC86C26E60220A4613EAD092365B278 e6ad3fd89023b050c8d4d1b3318608f7966fea86e3c35e2c95cca46ca5e860bf bffdc42f-42da-11f1-a4de-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43713 Institut Jožef Stefan 0 0 0