20.500.12556/DiRROS-29067 Effects of oral glucose tolerance test on microvascular and autonomic nervous system regulation in young healthy individuals Acute elevations in blood glucose can influence microvascular function and autonomic nervous system (ANS) reactivity, both implicated in cardiometabolic risk. We assessed the effects of oral glucose tolerance test (OGTT) on microvascular and ANS physiological responses in healthy young individuals. Using laser Doppler flowmetry (LDF), we measured basal skin microcirculatory blood flow and responses to post-occlusive reactive hyperemia, iontophoresis of acetylcholine (ACh), and sodium nitroprusside (SNP) in 28 participants before and 45 and 120 min after OGTT or water loading. LDF spectral components were analyzed using wavelet analysis (WA). ANS reactivity was evaluated from electrocardiogram recordings by analyzing heart rate variability (HRV). OGTT caused time-dependent changes in microvascular and HRV parameters. Endothelial nitric oxide-independent vasodilation transiently decreased during SNP response (p = 0.014), while myogenic component transiently increased (p = 0.029; two-way repeated measures ANOVA), with no significant change in the endothelial nitric oxide-dependent component. HRV measures RMSSD (p = 0.009) and SDNN (p = 0.008) decreased. Oral glucose loading affects microcirculation in healthy individuals, likely through modulation of endothelial nitric oxide-independent signaling, vascular smooth muscle responsiveness, and ANS reactivity. WA may offer a sensitive method for detecting microvascular dysfunction associated with physiological changes following oral glucose loading. acute hyperglycemia endothelial function heart rate variability microcirculation nitric oxide oral glucose loading oral glucose tolerance test wavelet analysis akutna hiperglikemija endotelijska funkcija variabilnost srčnega utripa mikrocirkulacija dušikov oksid oralna obremenitev z glukozo oralni test tolerance za glukozo analiza valov true false true Angleški jezik Slovenski jezik Neznano 2026-04-20 11:59:50 2026-04-20 11:59:50 2026-04-21 04:15:23 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 6. 10. 2025; str. 1-12 iss. 1, [article no.] 32858 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 612:616.4-053.2 2045-2322 10.1038/s41598-025-18209-1 251779587 RAZ_Kralj_Lana_2025.pdf RAZ_Kralj_Lana_2025.pdf 1 31F227EC3E01E1C3FB2E53E753653743 b9feda6eed89acdd6f62c19c1971acc56afecd6e98a0114bad5896325d774447 c66d0578-3c9f-11f1-92e1-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43408 https://www.nature.com/articles/s41598-025-18209-1 1 ad8aa126-3c9f-11f1-92e1-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43407 Univerzitetni klinični center Ljubljana 0 0 0