20.500.12556/DiRROS-29054 Computed tomography differentiation of compact and cancellous bone tissue in short and sesamoid bones Računalniškotomografska diferenciacija kompaktne in spongiozne kostnine pri kratkih in sezamoidnih kosteh Background: Selecting the most suitable skeletal remains for genetic analysis is challenging due to the variable DNA yield across different bone types and within individual bones. Compact bone typically preserves DNA longer, whereas cancellous bones, such as those in the hands and feet, often contain higher DNA quantities. This study aimed to incorporate dual-source computed tomography (DSCT), a technique frequently utilized for assessing bone density in living subjects, into targeted DNA sampling for dry, skeletonized remains by mapping compact and cancellous regions within six small skeletal elements. Materials and methods: A total of 137 bones were analysed using an imaging protocol specifically adapted to highlight the skeletal structure of small bones. This tailored protocol involved meticulous calibration of imaging parameters. Anatomical landmarks for six distinct elements were identified, and regions of interest were selected for bone density measurement in Hounsfield units (HU). Results: Among 461 assessed regions, 312 (68%) were classified as compact bone, and 149 (32%) as cancellous bone. Given the abnormal distribution of data, statistical differences were evaluated using 95% confidence intervals, with significance indicated by non-overlapping intervals. The analysis revealed statistically significant differences between compact and cancellous bone, as well as within each type across different bones. Conclusions: DSCT proved effective in mapping the internal structure of six small skeletal elements in dry, skeletonized remains, underscoring significant intra-bone variability in density. The findings illustrate DSCT's substantial potential for enhancing DNA sampling in forensic and paleogenetic studies, setting the stage for future research advancements. intro-bone variability compact and concellous bone dual-source CT short and sesamoid bones bone density variabilnost znotraj kosti kompaktna in gobasta kost CT z dvojinim virom kratke in sezamoidne kosti kostna gostota true false true Angleški jezik Slovenski jezik Neznano 2026-04-20 10:08:57 2026-04-20 10:08:58 2026-04-21 04:15:21 0000-00-00 00:00:00 2025 0 0 str. 311-318 no. 3 Vol. 59 Sep. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-07 1318-2099 10.2478/raon-2025-0022 233176067 RAZ_Gersak_Ziva_Miriam_2025.pdf RAZ_Gersak_Ziva_Miriam_2025.pdf 1 2B7033FDAFCC5B53188754608D36DD41 6d000e19716c658649c76aa275a84875f491d2e92995fb8c8bf7728a92cfb69e 4f053ff7-3c90-11f1-b8c8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43384 https://sciendo.com/article/10.2478/raon-2025-0022 1 3012a192-3c90-11f1-b8c8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43383 Univerzitetni klinični center Ljubljana 0 0 0