20.500.12556/DiRROS-28976 The association between periodontal inflamed surface area (PISA), inflammatory biomarkers, and mitochondrial DNA copy number Background/Objectives: Periodontitis is an inflammatory disease induced by bacteria in dental plaque that can activate the host’s immune-inflammatory response and invade the bloodstream. We hypothesized that a higher periodontal inflamed surface area (PISA) is associated with higher levels of inflammatory biomarkers, lower levels of antioxidants, and mitochondrial DNA copy number (mtDNAcn). Methods: Using periodontal parameters, we calculated the PISA score, measured the levels of inflammatory biomarkers and antioxidants in the serum, and took buccal swabs for mtDNA and nuclear DNA (nDNA) extraction. Results: Higher PISA was associated with higher CRP levels, higher leukocyte, neutrophil, and erythrocyte counts, and lower magnesium-to-calcium ratio, but not with mtDNAcn. A higher number of deep pockets was associated with higher leukocytes and neutrophil counts and higher uric acid levels. Conclusions: The PISA score might be an appropriate parameter to assess the inflammatory burden of periodontitis, but not to assess mitochondrial dysfunction after mtDNA isolation from buccal swabs. PISA mitochondrial DNA copy number mtDNA buccal swab oxidative biomarkers true false true Angleški jezik Ni določen Neznano 2026-04-15 13:03:19 2026-04-15 13:03:19 2026-04-16 04:06:21 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 8. 1. 2025; str. 1-19 iss. 1, [article no.] 24 Vol. 14 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 616.3 2077-0383 10.3390/jcm14010024 221396995 RAZ_Mance_Kristan_Romana_2025.pdf RAZ_Mance_Kristan_Romana_2025.pdf 1 343B01B6521884BE63290BB1694AB929 060254dd51dc2227daf1d3e64cae5eae383d323d0a44555f0132a0940d01b56a d761cd8c-38ba-11f1-9cc3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43243 https://www.mdpi.com/2077-0383/14/1/24 1 b932a301-38ba-11f1-9cc3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43242 Univerzitetni klinični center Ljubljana 0 0 0