20.500.12556/DiRROS-28914 Neuroactive compounds in tomatoes metabolic fate during in vitro digestion and colonic fermentation Neuroactive compounds in tomatoes: metabolic fate during in vitro digestion and colonic fermentation Neurodegenerative diseases are hard to treat, and their progression is linked to diet and the gut microbiota. Tomatoes contain potentially neuroactive compounds, but their fate during digestion and colonic fermentation remains unclear. This study tracked neuroactive compounds and fecal fatty acids (<C12) during in vitro gastrointestinal digestion and fecal fermentation. Conditions included a fecal blank, digested inulin, digested tomato, tomato with neuro-disrupting compounds, and individual neuroprotective compounds. Targeted analyses and linear mixed-effects models showed samples were primarily differentiated by tomato presence and fermentation time. Several neuroprotective compounds (e.g., tomatine, serotonin) remained stable (>50%, 24 h) or increased, whereas others (e.g., caffeic acid, rutin) degraded rapidly (<LOQ, 3 h). Neuro-disrupting compounds, including bisphenol S and difenoconazole, persisted largely unchanged (>80%, 24 h). Digested tomato enhanced acetic and propionic acid production, though attenuated by neuro-disruptors. The study clarifies colonic bioaccessibility of neuroactive compounds and their potential influence on the gut–brain axis. neuroactive compounds colon fecal fermentation targeted metabolomics bioaccesibility nevroaktivne sestavine črevesna fermentacija blata metabolomika biodostopnost true false true Elsevier Angleški jezik Slovenski jezik © 2026 The Authors. Neznano 2026-04-13 15:06:12 2026-04-13 15:06:12 2026-04-14 03:50:26 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji iz Slovenije: Urška Vrhovšek; Opis vira z dne 28. 1. 2026; str. [1-28] Vol. , [article no.] 148888 [in press] 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2026-01-15 2026-03-12 2026-03-18 543 1873-7072 10.1016/j.foodchem.2026.148888 272467203 https://www.sciencedirect.com/science/article/pii/S0308814626010460?via%3Dihub 1 8d32896e-3739-11f1-9cc3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43122 RAZ_Kovacic_Ana_2026.pdf RAZ_Kovacic_Ana_2026.pdf 1 865947350D72A7B1CD20BADD388979CC a0404bef2120debdf9b76a7be042463dae89fa1a2ebbc705b3469279cd5095ce cf9af0f0-3739-11f1-9cc3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43123 Institut Jožef Stefan 0 0 0