20.500.12556/DiRROS-28878 Telomere length, oxidative stress, and kidney damage biomarkers in Fabry nephropathy Fabry nephropathy is a life-threatening complication of Fabry disease characterized by complex and incompletely understood pathophysiological processes possibly linked to premature aging. We aimed to investigate leukocyte telomere length (LTL), oxidative stress, and kidney damage biomarkers in relation to kidney function. The study included 35 Fabry patients and 35 age and sex-matched control subjects. Based on the estimated slope of the glomerular filtration rate, the patients were divided into two groups. Relative LTL was quantified by qPCR, urinary biomarkers 8-hydroxy-2′ -deoxyguanosine (8-OHdG) and malondialdehyde (MDA) by UHPLC-MS/MS, and kidney damage biomarkers by flow cytometry. There was no statistically significant difference in LTL between Fabry patients and controls. However, a significant difference was observed in male patients compared to their matched control subjects (p = 0.013). Oxidative stress biomarkers showed no differences between patients and controls, while significant differences were observed in urinary IGFBP7, EGF, and OPN levels between Fabry patients with stable kidney function and those with progressive nephropathy (FDR = 0.021, 0.002, and 0.013, respectively). Significant differences were also observed in plasma levels of cystatin C, TFF3, and uromodulin between patients with progressive nephropathy and controls (all FDR = 0.039). Along with these biomarkers (FDR = 0.007, 0.017, and 0.010, respectively), NGAL also exhibited a significant difference between the two patient groups (FDR = 0.017). This study indicates accelerated telomere attrition, which may be related to disease burden in males. Furthermore, analyses of urinary oxidative stress markers revealed no notable disparities between the different kidney function groups, indicating their limited utility. However, promising differences were found in some biomarkers of kidney damage in urine and plasma. nephropathy telomere length oxidative stress kidney damage biomarker aging nefropatija dolžina telomera oksidativni stres poškodbe ledvic biomarker staranje true false true Angleški jezik Slovenski jezik Neznano 2026-04-10 11:50:10 2026-04-10 11:50:10 2026-04-11 03:54:49 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 4. 2. 2025; str. 1-15 no. 3 Vol. 14 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-056.7 2073-4409 10.3390/cells14030218 225070339 RAZ_Levstek_Tina_2025.pdf RAZ_Levstek_Tina_2025.pdf 1 A39139B615625DBE80FACD0EC4CAB68C 8c19e89b1a4fa110b9d51816e334e4d00cdd23a3a038b1d6ba7ebb496b2abebc d55185cc-34c2-11f1-b941-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43054 https://www.mdpi.com/2073-4409/14/3/218 1 ab7b319f-34c2-11f1-b941-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43053 Univerzitetni klinični center Ljubljana 0 0 0