20.500.12556/DiRROS-28814 Bacterial microbiome analysis of vaginal, cervical, and endometrial samples in patients with adenomyosis during the window of implantation Adenomyosis is a chronic gynecological condition affecting a substantial portion of women of reproductive age. With symptoms including abnormal uterine bleeding, chronic pelvic pain (CPP), dysmenorrhea, and infertility, poor response to symptomatic treatment and unfavorable outcomes of assisted reproductive technologies, it remains a significant diagnostic and therapeutic challenge. Here, we analyzed microbial compositions of the reproductive tract of women with adenomyosis (n = 33) in comparison with healthy controls (n = 31). Vaginal, cervical, and endometrial samples were collected using minimally invasive transcervical sampling techniques on the 22nd day of the menstrual cycle, during the so-called window of implantation. 16S rRNA was amplified and recorded using the next generation sequencing. Bioinformatic and statistical analysis focused on quantitative taxonomical characterization of the specimens’ microbiomes. Vaginal bacterial microbiome composition was consistent across the three anatomical sites. Compared with the control group, adenomyosis was associated with Lactobacillus iners, whereas Lactobacillus gasseri and Gardnerella vaginalis were negatively associated with adenomyosis and its clinical symptoms. G. vaginalis, typically considered a pathogen, was highlighted as an important dominant microbiome replacement for lactobacilli, more so in healthy women than in women with adenomyosis. Anaerococcus prevotii, Peptoniphilus grossensis, and Peptrostreptococcus anaerobius also showed weak correlation to adenomyosis. Differences in taxa abundance were detected in association with adenomyosis clinical symptoms. L. iners was associated with dysmenorrhea, heavy menstrual bleeding (HMB), as well as CPP. Prevotella disiens, Prevotella timonesis, and Dialister micraerophilus were associated with dysmenorrhea and Peptoniphilus grossensis with HMB, respectively. L. gasseri and L. jensenii appeared to anticorrelate with these symptoms. adenomioza mikrobiom rodil implantacijsko okno true false true Angleški jezik Slovenski jezik Neznano 2026-04-08 12:27:44 2026-04-08 12:27:45 2026-04-09 03:51:19 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Opis vira z dne 23. 2. 2026; str. 1-19 issue 4, [article no.] e02791-25 Vol. 14 18. feb. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61 2165-0497 10.1128/spectrum.02791-25 269348355 RAZ_Troha_Nika_2026.pdf RAZ_Troha_Nika_2026.pdf 1 F55FE4D4D9A86B654DE01BE425BF9F27 bdb6226024207e5541a77ae701380c43c3841a4a67b8a6e51e257fa1ce7c083c d0f575dd-3335-11f1-b941-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42927 https://journals.asm.org/doi/10.1128/spectrum.02791-25 1 97bf3436-3335-11f1-b941-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42926 Univerzitetni klinični center Ljubljana 0 0 0