20.500.12556/DiRROS-28781 Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings expert view of the European non-polio enterovirus network (ENPEN) Enteroviruses (EVs) are a common cause of a wide spectrum of infectious diseases, ranging from mild respiratory illnesses to severe neurological conditions, particularly affecting children. Current molecular methods, such as 5′UTR-based PCR for detection and (partial) VP1 gene sequencing for typing, are widely utilized. However, Next-Generation Sequencing (NGS), and bioinformatics offer a comprehensive alternative, enabling full-genome analyses for improved virus characterization, genomic epidemiological surveillance, and outbreak investigation. Despite its advantages, implementation of NGS poses challenges, particularly in standardizing and optimizing laboratory workflows (wet-lab) and bioinformatics analyses (dry-lab), methods that are not often readily accessible in many laboratories. Here, we discuss the potential of NGS as a tool for EV detection/characterization in clinical virology, public health, and research settings. We provide practical options for actions for implementing NGS to advance the understanding and management of enterovirus infections. These recommendations are based on expert discussions during the recent European non-polio enterovirus network (ENPEN) workshop held in Corfu, Greece, on 23–24 May 2024, aiming to guide harmonization of NGS practices across clinical, public health, and research settings. enteroviruses next generation sequencing viral metagenomics targeted PCR-based wet lab dry lab bioinformatics enterovirusi sekvenciranje naslednje generacije virusna metagenomika ciljno na PCR‑ju osnovano mokri laboratorij suhi laboratorij bioinformatika true false true Elsevier Angleški jezik Slovenski jezik Neznano 2026-04-07 11:13:19 2026-04-07 11:13:20 2026-04-08 03:47:41 0000-00-00 00:00:00 2026 0 0 Slovenski soavtor: Nataša Berginc Str. 1-10 št. članka 105940 Letn. 184 Jun. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-09-10 2026-03-22 2026-04-24 61:578 1386-6532 274121475 https://www.sciencedirect.com/science/article/pii/S1386653226000296?via%3Dihub 1 065f474a-3262-11f1-b7aa-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42855 benschop_next_generation_2026.pdf benschop_next_generation_2026.pdf 1 3B81DCD87B52ACC16F8CF3E7E2EDA8D0 7e8b2bc855922c79a55c6c8f470763bfb84c67beff583477e4fe4247e6be884f 4dc20a33-3262-11f1-b7aa-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42856 Nacionalni laboratorij za zdravje, okolje in hrano 0 0 0