20.500.12556/DiRROS-28763 CRISPR/dCas9-based systems: mechanisms and applications in plant sciences RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement. CRISPR interference CRISPR/dCas9 system crop improvement gene silencing RNAi transcriptional regulation CRISPR-interferenca sistem CRISPR/dCas9 izboljšanje pridelka utišanje genov RNAi regulacija transkripcije true false true Angleški jezik Slovenski jezik Neznano 2026-04-01 17:25:06 2026-04-01 17:25:07 2026-04-02 03:49:29 0000-00-00 00:00:00 2021 0 0 Soavtorji: Siti Nurfadhlina Mohd-Noor, Nadja Nolte, Boon Chin Tan; Nasl. z nasl. zaslona; Opis vira z dne 26. 3. 2026; 1-22 str. iss. 10, [article no.] 2055 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-09-29 277.2 2223-7747 10.3390/plants10102055 273252867 https://doi.org/10.3390/plants10102055 1 f8664e3d-2dde-11f1-9147-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42819 RAZ_Soong_Karlson_Chou_Khai_2021.pdf RAZ_Soong_Karlson_Chou_Khai_2021.pdf 1 49CB864B70A7FD21BD76BA0646C5A15B 7a744d9c4d773f5c69cc6d1c963428112bd5d11ddefe96e830f95f5a8e0d1942 1320eb79-2ddf-11f1-9147-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42820 Nacionalni inštitut za biologijo 0 0 0