20.500.12556/DiRROS-28632 Reversible thrombocytopenia of functional platelets after nose-horned viper envenomation is induced by a snaclec Profound and transient thrombocytopenia of functional platelets without bleeding was observed in patients envenomed by Vipera a. ammodytes (Vaa). This condition was rapidly reversed by administration of F(ab)2 fragments of immunoglobulin G targeting the whole venom, leaving platelets fully functional. To investigate the potential role of snake venom C-type lectin-like proteins (snaclecs) in this process, Vaa-snaclecs were isolated from the crude venom using different liquid chromatographies. The purity of the isolated proteins was confirmed by Edman sequencing and mass spectrometry. The antithrombotic effect was investigated by platelet agglutination and aggregation assays and blood coagulation tests. Using flow cytometry, the platelet activation and binding of Vaa-snaclecs to various platelet receptors was analyzed. Antithrombotic efficacy was tested in vivo using a mouse model of vascular injury. Two Vaa-snaclecs were purified from the venom. One of them, Vaa-snaclec-3/2, inhibited ristocetin-induced platelet agglutination. It is a covalent heterodimer of Vaa-snaclec-3 (α-subunit) and Vaa-snaclec-2 (β-subunit). Our results suggest that Vaa-snaclec-3/2 induces platelet agglutination and consequently thrombocytopenia by binding to the platelet receptor glycoprotein Ib. Essentially, no platelet activation was observed in this process. In vivo, Vaa-snaclec-3/2 was able to protect the mouse from ferric chloride-induced carotid artery thrombosis, revealing its applicative potential in interventional angiology and cardiology. reversible thrombocytopenia snaclec arterial occlusion kačji strup interventna kardiologija true false false Angleški jezik Slovenski jezik Neznano 2026-03-26 14:45:19 2026-03-26 14:45:19 2026-03-28 03:53:19 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 10. 9. 2024; str. 484-491 iss. 5 Vol. 125 May 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.9 2567-689X 10.1055/a-2408-9375 206918147 RAZ_Dobaja_Mojca_2025.pdf RAZ_Dobaja_Mojca_2025.pdf 1 9734B38989687BC598997C8E06956718 3d3a22cc0b9679cd34dc54b5490180fb61e69b1b24cc0f9f4e99a1969bc1958e 32805d39-291a-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42544 https://www.thieme-connect.com/products/ejournals/abstract/10.1055/a-2408-9375 1 09b261d5-291a-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42543 Univerzitetni klinični center Ljubljana 0 0 0