20.500.12556/DiRROS-28591 Complex vibroacoustic signalling during pair formation and courtship in the bushcricket Ancistrura nigrovittata (Orthoptera, Phaneropteridae) Many insects, including bushcrickets, use acoustic communication for mate finding and mate choice. These signals often produce simultaneous substrate vibrations, potentially aiding localization. However, independently produced vibratory signals are rarely documented in Orthoptera, despite their potential to enhance close-range communication. In the duetting bushcricket Ancistrura nigrovittata, acoustic communication has been extensively studied. We investigated whether any previously overlooked vibratory signals occur during close-range interactions in this species, and characterised their properties and interplay with acoustic signals. Combining laser vibrometry, audio and video recordings, we revealed soundless abdominal tremulation signals in both sexes during partner search and pair formation. Although subtle, these movements produce substrate vibration with amplitudes exceeding those induced by acoustic signalling (stridulation) by an order of magnitude. In both sexes, tremulation and acoustic signals can occur simultaneously or separately, demonstrating independent production mechanisms. Females combine song with tremulation during longdistance calling, use primarily tremulation after a male enters a plant, and typically cease signalling upon being found. Males add tremulation to the song after entering a plant and use it as the primary signal after partner contact. In both sexes, tremulation comprises mostly grouped pulses, emitted at a rate of about 2.5 Hz. Male tremulation exhibits a lower pulse number per train, higher dominant frequencies and amplitudes than female tremulation, and is typically accompanied by a short acoustic tick at the beginning of each pulse. Our findings reveal a previously unknown complexity of vibroacoustic communication in A. nigrovittata. tremulation vibration signal complex signal vibroacoustic duet vibracijski signal kompleksni signal tremulacija vibroakustični duet true false true Angleški jezik Slovenski jezik Neznano 2026-03-24 16:26:34 2026-03-24 16:26:34 2026-03-27 03:58:54 0000-00-00 00:00:00 2026 0 0 str. 1-17 Vol. 39, [article no.] 7 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-03-18 591.5 0892-7553 10.1007/s10905-026-09901-9 272308995 https://link.springer.com/article/10.1007/s10905-026-09901-9 1 dbbe273a-2795-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42459 RAZ_Stritih_Peljhan_Natasa_2026.pdf RAZ_Stritih_Peljhan_Natasa_2026.pdf 1 2791C018F80EE0F41FA209D15B267856 ce0b84a9ea2b357ebad336885f474874c39a71a42b74f85175f1c44c38211efd 041853bf-2796-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42460 Nacionalni inštitut za biologijo 0 0 0