20.500.12556/DiRROS-28564 Effect of male sex-category on gene expression in the m. longissimus dorsi (LD) and m. semispinalis capitis (SSC) expression profiling by high throughput sequencing Sus scrofa RNA- sequencing was performed to compare the effect of male sex category, i.e. entire males (EM), surgical castrates (SC), and immunocastrates (IC) on differential gene expression in LD and SSC muscles. Extracted RNA from 6 randomly selected animals of each sex category and muscle were pooled together and used for library preparation and sequencing. A total of 71 and 36 differentially expressed genes (DEGs) were detected in LD and SSC, respectively (|log2(fold change) >1| and -log10(q-value) <0.005). In LD, 36 DEGs were detected between EM and IC. 29 DEGs were detected between EM and SC and only 6 DEGs were detected when comparing IC and SC. In SSC, the number of DEGs was lower compared to LD. Only 8 DEGs were detected when either EM and IC or EM and SC were compared. The number of DEGs between IC and SC was higher for SCC than for LD (20 vs. 6). In LD, downregulated genes predominated in EM compared to either IC or SC and were mainly associated with skeletal muscle tissue development, skeletal cell differentiation, and negative regulation of biological/cellular processes. When comparing IC and SC, predominantly upregulated genes were observed, and the enriched GO terms were related to various biological processes, including regulation of fibrinolysis, apoptosis, energy homeostasis, fatty acid oxidation, and behaviour. In SSC, significantly enriched GO terms were found only for predominantly upregulated genes in IC compared to SC and were related to the regulation of energy homeostasis and fatty acid oxidation, response to hormones/stimuli, and regulation of cellular ketone metabolic process. prašiči RNK sekvence Krškopoljski prašič true false false Gene Expression Omnibus (GEO) Angleški jezik Slovenski jezik Neznano 2026-03-24 09:34:35 2026-03-24 09:34:36 2026-03-26 04:11:58 0000-00-00 00:00:00 2024 0 Bethesda 2024 Bethesda Nasl. z nasl. zaslona; Opis vira z dne 28. 3. 2025; ǂBioProject ǂPRJNA759775; GSE183240; 1 spletni vir 0000-00-00 Zaloznikova NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 230645251 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE183240 podatki na voljo po objavi 1 557983a9-275c-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42394 Kmetijski inštitut Slovenije 0 0 0