20.500.12556/DiRROS-28562 Transcriptome profiles of skeletal muscle in krškopolje and modern hybrid pigs genotype comparison and effects of dietary protein reduction Platforms (1) GPL26351 Illumina NovaSeq 6000 (Sus scrofa) RNA sequencing was performed to compare two pig breeds (the Slovenian Krškopolje breed and a modern commercial breed) and to evaluate the effect of breed specific dietary protein reduction on the transcriptomic profiles of two skeletal muscles: longissimus thoracis et lumborum (LD) and semispinalis capitis (SSC). Differentially expressed genes (DEGs) were identified using the criteria |log2FC| > 1 and q value < 0.005. The effect of dietary protein reduction was minimal in both breeds and muscles, with only small numbers of DEGs detected. In Krškopolje pigs receiving medium protein (MP) or low protein (LP) diets, no DEGs were identified. In contrast, in the modern breed, 10 DEGs were detected when comparing the high protein (HP) with the MP diet group. A similar pattern was observed in SSC, where 19 DEGs were detected in Krškopolje pigs (MP vs. LP) and 16 DEGs in the modern breed (HP vs. LP). In comparison, when the LD and SSC transcriptome profiles of the modern breed were compared with those of the Krškopolje breed, the number of DEGs was substantially higher:149 in LD and 201 in SSC. The DEGs in LD of modern breed reflect a shift toward faster growing, more glycolytic muscle with distinct immune and neuromuscular regulation. Likewise, the SSC of modern breed shows stronger activation of growth related and metabolic signaling pathways. RNA sequences pigs adipose tissue high throughput sequencing RNA transcriptome profiles gene expression locel breeds hybrid breeds data data set RNK sekvence prašiči režim prehranjevanja nivo proteinov v krmi histomorfološke lastnosti skeletno mišičan muskulatura visokozmogljivo sekvenciranje RNK profili transkriptomoov izražanje genov lokalne pasme sodobne pasme vsebnost beljakovin v prehrani podatki podatkovni set Sus scrofa true false true Gene Expression Omnibus (GEO) Angleški jezik Slovenski jezik Neznano 2026-03-24 09:06:29 2026-03-24 09:06:29 2026-03-26 04:11:35 0000-00-00 00:00:00 2026 0 Bethesda 2026 Bethesda Nasl. z. nasl. zaslona; Opis vira z dne 24. 3. 2026; 1 spletni vir 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-03-23 2030-03-23 636 272740099 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320088 Podatki nedostopni, 20. 3. 2026, dostopni po 20. 2. 2030 1 5ec51646-2758-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42389 RAZ_Vrecl_Milka_2026.pdf RAZ_Vrecl_Milka_2026.pdf 1 12F5E839ECC95EB69287C0B52E4FF85E da672460345aa3646fb9ab0197f0d57da967b0f7eb4eb78cdcaf146c103eaf8d 55c39850-2759-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42390 Kmetijski inštitut Slovenije 0 0 0