20.500.12556/DiRROS-28462 Adipose tissue transcriptome profiles of local Krškopolje pig and modern hybrid pigs receving reduced protein diets: expression profiling by high throughput sequencing Platforms (1), GPL26351 Illumina NovaSeq 6000 (Sus scrofa) The main objective of this study was to investigate transcriptomic differences in subcutaneous adipose tissue between the local Krškopolje pig and modern hybrids, and to assess the effects of a reduced-protein diet in both genotypes. Comparative analysis between Krškopolje pigs and modern crossbreeds revealed 375 differentially expressed genes, with 189 upregulated and 186 downregulated in Krškopolje pigs. The upregulated genes were enriched in processes related to adipogenesis (SLC7A10, ADIRF, INHBB, SFRP2), extracellular matrix remodeling (COL6A5, COL4A5, COL2A1), calcium signaling (TRPV4, CAMK2A, CALML5), pro-inflammatory cytokines (IL1A, TNFSF9, CXCL8, PTGS2), and cholesterol metabolism (CYP1A1, CYP2A19, CES1). In contrast, the reduced-protein diet induced only minor transcriptional changes at the individual gene level in both Krškopolje pigs and modern crossbreeds. RNA sequences pigs adipose tissue high throughput sequencing RNA transcriptome profiles gene expression locel breeds hybrid breeds data data set RNK sekvence prašiči maščobno tkivo visokozmogljivo sekvenciranje RNK profili transkriptomoov izražanje genov lokalne pasme sodobne pasme manjša vsebnost beljakovin v prehrani podatki podatkovni set Sus scrofa true false true Gene Expression Omnibus (GEO) Angleški jezik Slovenski jezik Neznano 2026-03-20 11:21:09 2026-03-20 11:21:09 2026-03-26 03:48:53 0000-00-00 00:00:00 2025 0 Bethesda 2025 Bethesda Nasl. z. nasl. zaslona; Opis vira z dne 7. 10. 2025; 1 spletni vir 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-09-24 2029-09-24 636 252017667 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309090 Podatki nedostopni, 7. 10. 2025, dostopni po 24. 9. 2029 1 86d2dda9-2446-11f1-b469-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42167 RAZ_Poklukar_Znidarsic_Klavdija_2025.pdf RAZ_Poklukar_Znidarsic_Klavdija_2025.pdf 1 49DF1579FA25210FE0E9DCF2986A2DCD 1a16a0dcaa758c497587d613d256e30119bb7eebb34ba5976399a328b7bfaf5d c13102a2-2446-11f1-b469-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42168 Kmetijski inštitut Slovenije 0 0 0