20.500.12556/DiRROS-28436 Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events Plant genetic engineering represents an important aspect of modern agriculture, and new geneticallymodified (GM) crop varieties are entering the market on a regular basis. This necessitates thedevelopment of high throughput multi-target analytical methods to detect and quantify theirpresence for regulatory compliance. In this study, we present a multiplex dPCR method for discrimi-native quantification of 19 GM soybean events and the lectin (Le1) endogene on a nanowell plate-based all-in-one dPCR system. The method consists of four 5-plex assays, taking advantage of theplatform’s multiple fluorescence detection channels. The assays complied with the minimum perfor-mance requirements in terms of specificity, trueness, precision, sensitivity and dynamic range,making them suitable for use in routine detection and quantification of GM crops. This methodrepresents the most comprehensive multi-target GM soybean quantification approach to date with-out the need for prior screening and features a simplified workflow, making it suitable for widespreadadoption. Our study sets a precedent for rapid and straightforward development of multiplex dPCRGM crop quantification assays to address the evolving demands of regulatory monitoring. genetically modified (GM) crops transgenic soybean Glycine max (L.) merr quantification digital PCR (dPCR) multiplex multi-target gensko spremenjene (gs) poljščine soja multipleks kvantifikacija digitalna PCR (dPCR) true false true Angleški jezik Slovenski jezik Neznano 2026-03-19 09:34:05 2026-03-19 09:34:05 2026-03-26 03:43:26 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: Dejan Štebih, Tina Demšar, David Dobnik; Opis vira z dne 2. 3. 2026; Str. 1-14 no. 1, [article no.] 2635816 Vol. 17 2026 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2026-02-27 577.2 2164-5701 10.1080/21645698.2026.2635816 270175747 https://www.tandfonline.com/doi/full/10.1080/21645698.2026.2635816#abstract 1 64c92408-236e-11f1-8070-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42094 RAZ_Jelencic_Amadej_2026.pdf RAZ_Jelencic_Amadej_2026.pdf 1 704A56AABA11400C1FC902A0D68FFF70 436e2033ce274138b75a8dc7e00f724566ec3ffd1d7e048aa76cb4dc4c756ae6 99a87da7-236e-11f1-8070-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42095 Nacionalni inštitut za biologijo 0 0 0