20.500.12556/DiRROS-28403 Piezostimulation using ultrasound-active poly-L-lactide films the effects of a ZnO filler and cold atmospheric plasma surface treatment Piezostimulation using ultrasound-active poly-L-lactide films: the effects of a ZnO filler and cold atmospheric plasma surface treatment Piezostimulation has the potential to promote human cell growth while inhibiting bacterial growth. Antimicrobial action physically destroys the bacterial cell wall without providing bacteria with too many options for developing resistance. However, the mechanism is typically not fast enough to follow the rapid kinetics of microbial growth, and improvement of the efficacy is needed. Here, we show that the synergy of bulk modification with a ZnO filler and surface modification with cold atmospheric plasma leads to a highly functional organic piezoelectric biomaterial. Specifically, the ZnO filler strengthens the piezoelectric response of piezoelectric poly-L-lactide (piezo-PLLA) after its integration into the polymer bulk, whereas the plasma surface treatment changes the surface chemistry and partially reveals integrated ZnO particles on the surface. Both effects significantly affected piezostimulation and improved the interactions with two types of cells. Specifically, the increased content of ZnO NPs in the piezo-PLLA films increased the contact of both bacteria (E. coli) and human keratinocytes (HaCaT) with the film surface. Consequently, after activation with ultrasound (1 MHz), the surface very effectively transferred the signal to the adhered cells, which resulted in fast and effective antimicrobial activity. Moreover, for adhered HaCaT cells, stimulation promoted proliferation, cell mobility, intercellular connections and cytoskeleton formation. In combination, these two types of interactions are very important for promoting skin regeneration and wound healing. piezostimulation antimicrobial surfaces poly-L-lactide films piezoelektrična stimulacija protimikrobni materiali true false true The Royal Society of Chemistry Združeno kraljestvo Angleški jezik Slovenski jezik This journal is © The Royal Society of Chemistry 2026 Neznano 2026-03-18 13:09:05 2026-03-18 13:09:06 2026-03-24 04:00:08 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: Martina Žabčić, Lea Gazvoda, Masoumeh Sepideh Salehidashtbayaz, Ita Junkar, Tina Radošević, Matejka Podlogar, Martin Šala, Matjaž Spreitzer, Marija Vukomanović; Opis vira z dne 18. 3. 2026; str. [1-18] iss. Vol. 14 [in press] 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-10-09 2026-01-31 2026-02-03 621.7+621.9 2047-4849 10.1039/D5BM01497B 272105219 https://pubs.rsc.org/en/content/articlelanding/2026/bm/d5bm01497b 1 4645ae7c-22c3-11f1-a33f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42006 RAZ_Stor_Spela_2026.pdf RAZ_Stor_Spela_2026.pdf 1 86016B14A45E175F5D06EE85D61BB011 dc2f940436babef9915955cf3cbe0762274735596fc64fe789d8b851a2818e86 c66113d5-22c3-11f1-a33f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42007 Institut Jožef Stefan 0 0 0