20.500.12556/DiRROS-28401 Genetic testing for monogenic forms of male infertility contributes to the clinical diagnosis of men with severe idiopathic male infertility In recent years, many genes have been associated with male infertility; however, testing of monogenic forms has not yet been clinically implemented in the diagnosis of severe forms of idiopathic male infertility, as the diagnostic utility has not been established yet. The aim of this study was therefore to answer if the implementation of genetic testing for monogenic forms of male infertility could contribute to the clinical diagnosis of men with severe forms of idiopathic male infertility. Materials and Methods: Based on the ClinGene curation protocol, we defined a panel of genes with sufficient evidence for the involvement with severe male infertility. We tested the 21-gene panel in a representative multicentric cohort of men with significantly impaired spermatogenesis. We performed whole exome sequencing on 191 infertile men with severe forms of idiopathic male infertility; non-obstructive azoospermia, and severe oligozoospermia (<5 million spermatozoa/mL). The control group consisted of 216 men who fathered a child. DNA was prepared based on the Twist CORE exome protocol and sequenced on the Illumina NovaSeq 6000 platform. Variants were classified using the Association for Clinical Genomic Science (ACGS) Best Practice Guidelines for Variant Classification in Rare Disease 2020. Results: We identified potential monogenic disease-causing variants in four infertile men. Pathogenic/likely pathogenic variants in STAG3 (c.2776C>T, p.Arg926*; c.2817delG, p.Leu940fs), MSH4 (c.1392delG, p.Ile465fs; c.2261C>T, p.Ser754Leu), TEX15 (c.6848_6849delGA, p.Arg2283fs; c.6271dupA, p.Arg2091fs), and TEX14 (c.1021C>T, p.Arg341*) genes were found. Conclusions: In the present multicentric cohort study, a monogenic cause in 2.1% of infertile men was identified. These findings confirm the utility of monogenic testing and suggest the clinical use of monogenic testing for men with severe forms of idiopathic male infertility. azoospermia genetic testing male meiosis true false true Angleški jezik Ni določen Neznano 2026-03-18 12:22:16 2026-03-18 12:22:17 2026-03-24 03:59:29 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 21. 5. 2025; str. 908-917 no. 4 Vol. 43 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.6:575 2287-4690 10.5534/wjmh.240149 236589059 RAZ_Podgrajsek_Rebeka_2025.pdf RAZ_Podgrajsek_Rebeka_2025.pdf 1 13CEEF866D60254DDDBD6813CD241654 d02ac3ee17a2792f704c97438bec7bddebbe5713ad42ab9d03ed9b1dbff4430e f0837f1e-22bc-11f1-a33f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42003 https://wjmh.org/DOIx.php?id=10.5534/wjmh.240149 1 bc740d7e-22bc-11f1-a33f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42002 Univerzitetni klinični center Ljubljana 0 0 0