20.500.12556/DiRROS-28060 Proteomic analysis identifying proteins relevant for treatment success following experimental neonatal inflammationsensitized hypoxia-ischemia BACKGROUND: Understanding the mechanisms of injury following neonatal hypoxic-ischemic encephalopathy (HIE) is a major goal in neonatal research. HIE can have severe effects on cognitive and motor development in newborns, including an increased risk of death. As the incidence is 10–20 times higher in low- and middle-income countries compared to developed countries, the interest in a therapy exists worldwide. Therapeutic hypothermia (HT) is the only effective treatment after HIE. However, TH is not universally effective, particularly in cases of inflammation-sensitized hypoxia-ischemia (HI); it provides limited benefit. METHODS: To identify proteins that may contribute to the reduced efficacy of HT in the case of pre-HI inflammation sensitization, the proteomic profiles of animals subjected to HI and HT combined with lipopolysaccharide (LPS) were analyzed via liquid chromatography mass spectrometry (LC-MS/MS). RESULTS: We identified proteins that potentially support the efficacy of HT and those that prevent the success of the therapy in the neonatal rat model of inflammation-sensitized HI. CONCLUSION: This study represents a step forward in identifying proteins related to the efficacy of HT following inflammationsensitized HI. hipoksija beljakovine novorojenčki proteomika true false true Angleški jezik Slovenski jezik Neznano 2026-03-09 11:17:48 2026-03-09 11:17:48 2026-04-23 12:56:00 0000-00-00 00:00:00 2026 0 0 str. 1591–1601 Vol. 99 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-053.2 0031-3998 10.1038/s41390-025-04097-8 237965315 RAZ_Burkard_Hannah_2025.pdf RAZ_Burkard_Hannah_2025.pdf 1 137058DD24AE691157938E0D588CEB26 5334daefe69b4e9172436075b45cf66f2b16cc1055eae20c62c501a23e77ea02 5bf7b883-1ba1-11f1-b779-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41432 https://www.nature.com/articles/s41390-025-04097-8 1 3b559534-1ba1-11f1-b779-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41429 Univerzitetni klinični center Ljubljana 0 0 0