20.500.12556/DiRROS-28026 The FORGENIUS genomic resources new genotyping tools and genomic data for 23 forest tree species and their genetic conservation units Genetic diversity is a critical but often overlooked component of biological diversity. The European H2020 FORGENIUS project is aimed at increasing the quality and quantity of genetic data to start monitoring the European network of forest Genetic Conservation Units (GCUs). A first step in this direction was developing standardised genomic resources for 23 forest tree species, spanning from rare and scattered (e.g., Abies nebrodensis and Torminalis glaberrima) to widespread and stand-forming, economically relevant ones (e.g., Fagus sylvatica, Picea abies and Pinus sylvestris). Here, we describe the development and application of targeted genotyping tools, primarily based on Single Primer Enrichment Technology (SPET), along with existing SNP arrays for the selected species. The SPET panels developed in FORGENIUS were designed to capture ⁓10,000 loci per species, balancing species-specific and randomly distributed regions to ensure broad genome coverage and minimise ascertainment bias. Across 7220 genotyped trees, we identified over 1.8 million single nucleotide polymorphisms (SNPs) covering approximately 50 Mb of DNA sequence. SPET panels demonstrated high genotyping efficiency and cross-species transferability, especially within genera such as Quercus and Abies. They represent a cost-effective, flexible, and scalable solution for population-level genetic assessments across diverse taxa, enabling standardised, genome-wide characterisation of the GCU network. These resources not only promote the establishment of genetic monitoring, support genetically informed conservation strategies and improve our understanding of adaptive responses in European forests, but also enhance species delimitation and hybrid detection, and enable the characterisation of phylogenetically related but previously underexplored species. forest tree species genetic conservation units genetic diversity genetic monitoring single primer enrichment technology targetedgenotyping SNP SPET gozdne drevesne vrste gozdni genski rezervat SNP SPET true false true Angleški jezik Slovenski jezik Znanstveno delo 2026-03-06 11:07:19 2026-03-06 11:07:20 2026-03-07 03:51:43 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Opis vira z dne 5. 3. 2026; Skupno št. avtorjev: 78; str. 1-16 iss. 3 [article no. e70115] Vol. 26 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*1 1755-0998 10.1111/1755-0998.70115 270742019 https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.70115 1 45598789-1944-11f1-81cd-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41350 Molecular_Ecology_Resources_-_2026_-_Pinosio_-_The_FORGENIUS_Genomic_Resources__New_Genotyping_Tools_and_Genomic_Data_for.pdf Molecular_Ecology_Resources_-_2026_-_Pinosio_-_The_FORGENIUS_Genomic_Resources__New_Genotyping_Tools_and_Genomic_Data_for.pdf 1 8C5ABBF501F804AE79B8F739C4628B3A cee9f6198922f9e48c1e0c8d7983d999bf80d5dbcd156c315180a5e2c938f92d 618aa4b6-1944-11f1-81cd-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41351 Gozdarski inštitut Slovenije 0 0 0