20.500.12556/DiRROS-27947 Exploring the genetic diversity of recent Ralstonia pseudosolanacearum phylotype I findings in Europe The increasing prevalence of Ralstonia pseudosolanacearum phylotype I (phy I) in agricultural regions across Europe highlights the need for heightened awareness and preventive measures. Recent detections in multiple European countries span a range of agricultural systems, host plants, and natural aquatic environments. These findings have been linked either to infected plant material at import (interceptions) or to plants cultivated in specific European regions, often after importation of infected plant material. To investigate the genetic diversity of these emerging populations, we analysed whole-genome sequence (WGS) data from a collection of 62 R. pseudosolanacearum phy I isolates, including those recently obtained in various European countries, using average nucleotide analysis (ANI) and core genome multilocus sequence typing (cgMLST). ANI analysis revealed two distinct major clades: the "rose clade", comprising isolates from rose, potato, surface water, and bittersweet, most closely matching with sequevar 33, and the "major ginger clade", including isolates from members of the Zingiberaceae family, with the best match to sequevar 30. Additionally, several minor clades of Zingiberaceae isolates and a "tomato clade” (most closely related to sequevar 18) were identified. Pathogenicity assays with high inoculation titers were performed on tomato to assess the biological relevance of the observed genetic diversity. Our findings suggest multiple independent introduction events of virulent R. pseudosolanacearum phy I populations into Europe between 2015 and 2024. Given the genetic variability of the introduced bacterial isolates, the pathogen’s ability to systemically infect a broad range of host plants which can differ per isolate, and its persistence in diverse agricultural and environmental niches, the implementation of stringent import control measures for plant material entering the European Union is strongly recommended. Ralstonia pseudosolanacearum surface water Zingiberaceae Solanaceae Rosaceae Ralstonia pseudosolanacearum površinska voda Zingiberaceae Solanaceae Rosaceae true false true Angleški jezik Slovenski jezik Neznano 2026-03-02 09:24:23 2026-03-02 09:24:23 2026-03-03 03:52:35 0000-00-00 00:00:00 2026 0 0 Avtorji: Maria Bergsma-Vlami, Eva Fornefeld, Rene Glenz, Anna Kołodziejska, Francesca Giacobbi, Rossella Gozzi, Stefania Loreti, Ágnes Ambrus, D. Hederics, Christophe Debonneville, Tanja Dreo, Manca Pirc, Aleksander Benčič, Jolien Venneman, Johan Van Vaerenbergh, Steve Baeyen, Maria Holeva, P.E. Glynos, Robert Vreeburg, Tijs van den Bosch, Chiel Pel, Tom Raaymakers; str. [1-15] Vol. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-02-10 632.4:579.8 0929-1873 10.1007/s10658-026-03191-9 268258051 https://link.springer.com/article/10.1007/s10658-026-03191-9 1 3a55bddc-1611-11f1-8c73-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41167 RAZ_Bergsma-Vlami_Maria_2026.pdf RAZ_Bergsma-Vlami_Maria_2026.pdf 1 E7C4F26CC562F7470AAAE0CEC8DCA13B 24b78ac4bb9b0786798455f03f081111fefc87b31d207d9b440fb31887db1fb7 9379029a-1611-11f1-8c73-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41168 Nacionalni inštitut za biologijo 0 0 0