20.500.12556/DiRROS-27892 Morpho-anatomical properties and terpene composition of Picea Omorika (Pančić) Purk. needles from Bosnia and Herzegovina first_pagesettingsOrder Article Reprints Open AccessArticle Morpho-Anatomical Properties and Terpene Composition of Picea Omorika (Pančić) Purk. Needles from Bosnia and Herzegovina by Biljana M. Nikolić 1,*ORCID,Zorica S. Mitić 2ORCID,Dalibor Ballian 3,4,5ORCID,Marina M. Todosijević 6ORCID,Jelena S. Nikolić 2ORCID,Stefan Ivanović 7ORCID andVele V. Tešević 6ORCID 1 Institute of Forestry, Department of Genetics, Plant Breeding, Seed and Nursery Production, Kneza Višeslava 3, 11000 Belgrade, Serbia 2 Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia 3 Faculty of Forestry, University of Sarajevo, Zagrebačka 20, 71000 Sarajevo, Bosnia and Herzegovina 4 Department of Forest Physiology and Genetics, Slovenian Forestry Institute, Večna pot 2, 1000 Ljubljana, Slovenia 5 Academy of Sciences and Arts of Bosnia and Herzegovina, Bistrik 7, 71000 Sarajevo, Bosnia and Herzegovina 6 Faculty of Chemistry, University of Belgrade, Studentski trg 12–16, 11000 Belgrade, Serbia 7 Laboratory of Chemistry, Technology and Metallurgy, Institute for Chemistry, University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia * Author to whom correspondence should be addressed. Forests 2025, 16(5), 791; https://doi.org/10.3390/f16050791 Submission received: 1 April 2025 / Revised: 6 May 2025 / Accepted: 7 May 2025 / Published: 8 May 2025 (This article belongs to the Special Issue Specialized Metabolites and Structure of Woody Plants) Downloadkeyboard_arrow_down Browse Figures Review Reports Versions Notes Abstract Picea omorika (Pančić) Purk., (Serbian spruce) is a relic, endemic, and vulnerable conifer that remains insufficiently studied to date. To the best of our knowledge, this is the first report on the morpho-anatomical and phytochemical diversity of needles from three populations in Bosnia and Herzegovina. The length of two-year-old needles was measured with a digital caliper. The next six properties were measured based on cross-sections of the needles using a light microscope. An analysis of volatile compounds was carried out using gas chromatography coupled with mass spectrometry (GC-MS) and flame ionization detection (GC-FID). The highest values of needle traits were found in the Viogor population, with the lowest in the Tisovljak population, which was statistically confirmed. There was also a significant difference between needles from Bosnia and Herzegovina and those from Serbia. Bornyl acetate, camphene, limonene, and α-pinene were identified as the major terpene compounds. Multivariate analyses also suggested a tendency toward the separation of the Tisovljak population. A statistical comparison of three Bosnian and Herzegovinian and four Serbian populations (previously studied and published) revealed two distinct groups: (1) three Bosnian populations and the Vranjak population from Serbia, and (2) three populations from Serbia—Štula, Zmajevački Potok, and Mileševka Canyon. The general conclusions are that divergence in needle morpho-anatomy aligns with divergence in needle chemistry and that Bosnian and Herzegovinian populations are distinct from nearly all Serbian populations. Picea omorika Serbian spruce population morpho-anatomy terpenes multivariate statistics Picea omorika srbska smreka razširjenost morfoanatomija multivariatna statistika true false true Angleški jezik Slovenski jezik Znanstveno delo 2026-02-26 15:15:01 2026-02-26 15:15:02 2026-02-27 03:58:49 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 26. 2. 2026; str. 1-15 iss. 5 Vol. 16 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*18 1999-4907 10.3390/f16050791 269811203 https://www.mdpi.com/1999-4907/16/5/791 1 8be1c3af-131d-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41054 forests-16-00791-v3.pdf forests-16-00791-v3.pdf 1 1C80942BAD67B592965A8263A7467EF1 b98dd31b7b083e02b60c98ccbe2df3a35f4a23d572ec1681bd5a1209fd57e320 9968edbb-131d-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=41055 Gozdarski inštitut Slovenije 0 0 0