20.500.12556/DiRROS-27827 Patient endothelial colony-forming cells to model coronary artery disease susceptibility and unravel the role of dysregulated mitochondrial redox signalling Mechanisms involved in the individual susceptibility to atherosclerotic coronary artery disease (CAD) beyond traditional risk factors are poorly understood. Here, we describe the utility of cultured patient-derived endothelial colony-forming cells (ECFCs) in examining novel mechanisms of CAD susceptibility, particularly the role of dysregulated redox signalling. ECFCs were selectively cultured from peripheral blood mononuclear cells from 828 patients from the BioHEART-CT cohort, each with corresponding demographic, clinical and CT coronary angiographic imaging data. Spontaneous growth occurred in 178 (21.5%) patients and was more common in patients with hypertension (OR 1.45 (95% CI 1.03–2.02), p = 0.031), and less likely in patients with obesity (OR 0.62 [95% CI 0.40–0.95], p = 0.027) or obstructive CAD (stenosis > 50%) (OR 0.60 [95% CI 0.38–0.95], p = 0.027). ECFCs from patients with CAD had higher mitochondrial production of superoxide (O2−–MitoSOX assay). The latter was strongly correlated with the severity of CAD as measured by either coronary artery calcium score (R2 = 0.46; p = 0.0051) or Gensini Score (R2 = 0.67; p = 0.0002). Patient-derived ECFCs were successfully cultured in 3D culture pulsatile mini-vessels. Patient-derived ECFCs can provide a novel resource for discovering mechanisms of CAD disease susceptibility, particularly in relation to mitochondrial redox signalling. coronary artery disease biomarkers endothelial cells risk factors atherosclerosis bolezen koronarnih arterij biomarkerji endotelijske celice dejavniki tveganja aterosklerozae true false true Angleški jezik Slovenski jezik Neznano 2026-02-25 15:04:53 2026-02-25 15:04:54 2026-02-26 03:55:16 0000-00-00 00:00:00 2021 0 0 Nasl. z nasl. zaslona; Opis vira z dne 11. 12. 2025; str. 1-17 iss. 10, [article no.] 1547 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.1 2076-3921 10.3390/antiox10101547 260955651 RAZ_Besnier_Marie_2021.pdf RAZ_Besnier_Marie_2021.pdf 1 EE025E593C29B252B98E6A3D16F0B642 4ec4edd2acf7be8025066a6388a8391f9c685a726a413f36a2500d683fdeff92 a7db4bac-1253-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40918 https://doi.org/10.3390/antiox10101547 0 b34df7c9-1253-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40919 Univerzitetni klinični center Ljubljana 0 0 0