20.500.12556/DiRROS-27758 Modulation of contactless high intensity pulsed electromagnetic field induced electroporation and gene delivery efficacy using various nanoparticles Introduction: High-intensity pulsed electromagnetic fields (HI-PEMF) can be used to trigger contactless permeabilization of the plasma membrane similar to electroporation (EP). The permeabilization efficiency and gene delivery by HI-PEMF in vitro are currently inferior to EP. It was suggested that the methodology can be improved with conductive gold nanoparticles (AuNPs), which are reported to amplify the induced electric field in close proximity to the cell membrane. Objectives: Therefore, in this work, we have studied different NPs, which varied in material/conductivity (gold and silica), size (10–50+ nm), shape (i.e., round and rods), concentration (50–200 µg/mL), and functionalization (pegylated or not), and combined them with HI-PEMF (6.7 T × 100 pulses, 1 Hz). Methods: The normal Chinese hamster ovary cell line (CHO) and the cancer human urinary bladder’s transitional carcinoma cell line (T24) were used as a model. We have characterized cell membrane permeabilization using propidium iodide (PI) and the efficacy of gene delivery using pEGFP-N1. Results: Larger NPs and higher NP concentrations resulted in up to a 10% increase in membrane permeability. In contrast, semispherical and rod-shaped AuNPs did not further enhance permeabilization efficiency. Gene delivery efficiency increased from 3% in control samples to 6% in the presence of 50 nm AuNPs. Overall, CHO cells were more susceptible to HI-PEMF-induced effects than T24 cells. Conclusions: This study shows the potential to increase gene delivery efficacy by combining HI-PEMF treatment with conductive NPs. However, it was concluded that the HI-PEMF-induced effects are highly dependent on the cell line, NP type, and concentration and therefore require further investigation. gene delivery dostavni sistemi za gene true false false Mary Ann Liebert ZDA Angleški jezik Slovenski jezik Users who receive access to an article through a repository are reminded that the article is protected by copyright and reuse is restricted to non-commercial and no derivative uses. Neznano 2026-02-24 12:17:30 2026-02-24 12:17:30 2026-02-25 03:50:46 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji iz Slovenije: Matej Kranjc, Slavko Kralj, Maja Caf, Rok Romih, Samo Hudoklin, Damijan Miklavčič; Opis vira z dne 19. 2. 2026; str. 1-11 iss. Vol. 8 [in press] 2026 0000-00-00 PreprintVObjavi Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-02-13 577 2576-3113 10.1177/25763113261423900 269029379 https://journals.sagepub.com/doi/10.1177/25763113261423900 1 6be81c1b-1172-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40794 RAZ_Polajzer_Tamara_2026.pdf RAZ_Polajzer_Tamara_2026.pdf 1 F9298B35B3212BE8C40EB95E7C287037 214cd3c7013ee724fb3ef8362d7a786109d16b9878ad1e0c7afc622db274cb5e 03f745a8-1173-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40797 Institut Jožef Stefan 0 0 0