20.500.12556/DiRROS-27731 Microbial diversity in drug-naïve Parkinson’s disease patients Parkinson’s disease (PD) is a neurological disorder characterized by rigidity, bradykinesia and tremor. Several genetic and environmental causes of PD are known, and there is emerging evidence of the possible contribution of the gut microbiome to the disease onset, severity, and response to therapy. While previous research has shown several differences in the microbiome of PD patients under therapy as opposed to healthy controls, few prospective studies have included drug-naïve patients. In order to evaluate the gut microbiome composition prior to therapy initiation, we collected and performed 16S rRNA gene sequencing of the stool samples from 49 drug-naïve PD patients and compared them to 34 diet and lifestyle-matched controls from the Croatian population (GiOPARK Project). While no significant alpha diversity difference was observed between the patients and controls, the differential relative abundance analysis showed an increase in Bacteroides fluxus, B. interstinalis, B. eggerthii, and Dielma fastidiosa in the drug-naïve PD patients compared to controls, while Alistipes, Barnesiella and Dialister spp. were decreased in patients compared to controls. Despite preserved overall diversity, these changes may indicate early microbial dysbiosis and represent a foundation for future studies exploring microbiome changes across disease progression and treatment. Parkinson’s disease neurological disorder gut microbiome microbial diversity microbial dysbiosis true false true Angleški jezik Ni določen Neznano 2026-02-23 14:38:01 2026-02-23 14:38:02 2026-02-24 04:02:01 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne20. 8. 2025; str. 1-16 iss. 8, [article no.] e0328761 Vol. 20 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.8:579.2 1932-6203 10.1371/journal.pone.0328761 246046979 RAZ_Papic_Elisa_2025.pdf RAZ_Papic_Elisa_2025.pdf 1 18A609848D7523E9312DCBBE05B6DBD7 61e01858cdfb7a25cf920bebcca78415d973b8b0a18f0e1f2ab2bd7746374ed4 17fa42f6-10bd-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40740 https://doi.org/10.1371/journal.pone.0328761 0 2501e4b6-10bd-11f1-9cb0-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40741 Univerzitetni klinični center Ljubljana 0 0 0