20.500.12556/DiRROS-27655 Integrative profiling of condensation-prone RNAs during early development Complex RNA-protein networks play a pivotal role in the formation of many types of biomolecular condensates. How RNA features contribute to condensate formation, however, remains incompletely understood. Here, we integrate tailored transcriptomics assays to identify a distinct class of developmental condensation-prone RNAs termed “smOOPs” (semi-extractable, orthogonal-organic-phase-separation-enriched RNAs). These transcripts localize to larger intracellular foci, form denser RNA subnetworks than expected, and are heavily bound by RNA-binding proteins (RBPs). Using an explainable deep learning framework, we reveal that smOOPs harbor characteristic sequence composition, with lower sequence complexity, increased intramolecular folding, and specific RBP-binding patterns. Intriguingly, these RNAs encode proteins bearing extensive intrinsically disordered regions and are highly predicted to be involved in biomolecular condensates, indicating an interplay between RNA- and protein-based features in phase separation. This work advances our understanding of condensation-prone RNAs and provides a versatile resource to further investigate RNA-driven condensation principles. condensation-prone RNAs RNA-RNA interactions phase separation biokemija beljakovine RNK zaporedja interakcije med RNK in RNK true false true Angleški jezik Slovenski jezik © 2025 The Author(s). Published by Elsevier Inc. Neznano 2026-02-17 13:20:21 2026-02-17 13:33:43 2026-02-18 03:58:07 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji iz Slovenije: Jona Novljan, Boštjan Kokot, Iztok Urbančič, Jernej Ule, Miha Modic; Opis vira z dne 28. 11. 2025; str. 1-16, e1-e9 iss. 2, [article no.] 101065 Vol. 6 11 Feb. 2026 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2026-02-11 577 2666-979X 10.1016/j.xgen.2025.101065 259148291 https://www.sciencedirect.com/science/article/pii/S2666979X25003210 1 099360ec-0bfb-11f1-bcbc-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40573 RAZ_Klobucar_Tajda_2026.pdf RAZ_Klobucar_Tajda_2026.pdf 1 71C6CCDFE200FA89EF16450199B6391A 7aa490611293f215183fd17c8ac1544613f20a484eb93a13d44e84e8c00f9f98 b6ed47b9-0bfc-11f1-bcbc-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40574 Kemijski inštitut 0 0 0