20.500.12556/DiRROS-27638 Advanced LC-MS/MS methodologies for the analysis of neuroactive compounds in tomato-based diets insights from 2D in vitro digestion models Advanced LC-MS/MS methodologies for the analysis of neuroactive compounds in tomato-based diets: insights from 2D in vitro digestion models The digestive fate of neuroactive food compounds remains poorly understood despite growing interest in their role in brain health. This study developed advanced analytical methods to identify neuroprotective and neuro-disrupting compounds in tomatoes before and after digestion and following fecal fermentation. Using in vitro digestion and fermentation models, we characterized compound behavior during digestion. Most compounds were quantified in undigested tomatoes. Digestion revealed diverse outcomes: some compounds transformed (e.g., chlorogenic acids), others produced (e.g., linoleic acid), and several remained stable with potential to reach the colon (e.g., serotonin, rutin). Notably, neuro-disrupting compounds like bisphenols remained largely unaltered and bioaccessible in the colon. Fecal fermentation further showed dynamic changes, with some compounds decreasing (e.g., rutin) and others increasing (e.g., quercetin). These findings provide both methodological advances and mechanistic insights into neuroactive compound behavior during digestion, informing future research on gut-mediated dietary effects in the context of neurodegenerative disease prevention. neuroactive compounds fecal fermentation target analysis bioaccesibility nevroaktivne sestavine fermentacija blata ciljna analiza true false true Elsevier Nizozemska Angleški jezik Slovenski jezik © 2026 The Authors. Neznano 2026-02-17 10:06:52 2026-02-17 10:06:53 2026-02-18 03:58:03 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji iz Slovenije: Urška Vrhovšek; Opis vira z dne 28. 1. 2026; str. 1-25 Vol. , [article no.] 101231 [in press] 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-11-16 2026-01-20 2026-01-20 543 2772-753X 10.1016/j.focha.2026.101231 266449923 https://www.sciencedirect.com/science/article/pii/S2772753X26000146?via%3Dihub 1 08ed0101-0be0-11f1-bcbc-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40538 RAZ_Kovacic_Ana_2026.pdf RAZ_Kovacic_Ana_2026.pdf 1 6D31D06AA67A0650EE61BB80A58D1EAF 6d97da9dc69002b995a1028e7e08836e5b9a1b9f0e38284899e0f6486731b1cb b77c1afe-0be0-11f1-bcbc-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40539 Institut Jožef Stefan 0 0 0