20.500.12556/DiRROS-27565 Co-expression of holin gene improves heterologous cellulase secretion and surface display by lactic acid bacteria Lactococcus cremoris Genetically modified Lactococcus cremoris strains producing heterologous cellulases emerge as promising candidates for cost-effective bioprocessing of plant biowaste into high-value organic compounds. A key challenge in enabling efficient cellulose degradation by L. cremoris remains the effective secretion of heterologous cellulases. A potential solution would be to use genetic engineering to enhance the permeabilization of bacterial cell membrane and cell wall to facilitate the release of cellulases. This could be achieved by the co-expression of cellulase genes with genes encoding the prophage lytic proteins such as holin and endolysin. genetic engineering lytic proteins Lactococcus cremoris genski inžiniring plazemske beljakovine bakterije true false true Springer Nature Švica Angleški jezik Slovenski jezik © The Author(s) 2026. Neznano 2026-02-13 09:21:36 2026-02-13 09:21:37 2026-02-14 04:04:00 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Opis vira z dne 28. 1. 2026; str. 1-49 iss. Vol. [in press] 2026 0000-00-00 Zaloznikova Objavljeno apc 2025-11-03 2026-01-12 2026-02-12 60 1754-1611 10.1186/s13036-026-00625-0 266410499 https://link.springer.com/article/10.1186/s13036-026-00625-0 1 061597dc-08b5-11f1-bcbc-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40380 RAZ_Stravs_Petra_2026.pdf RAZ_Stravs_Petra_2026.pdf 1 21A6235F307514A47B719DB840E09D64 79a87d286ffb82307c1cedd26da48ed82775912fc59882e1bce683a59dc3ab18 e98b2616-08b5-11f1-bcbc-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40382 Institut Jožef Stefan 0 0 0