20.500.12556/DiRROS-27561 DNA Damage Induction After Exposure to Ferrite Nanoparticles for biomedical applications (MxFe3-xO4, M = Fe, Zn, Mn) in an Advanced 3D Human Hepatic in vitro Model version v1 Nanosized spinel-type ferrites have gained recognition as a unique class of engineered nanomaterials with promising applications, but their safety profiles remain insufficiently explored. Although iron (Fe), zinc (Zn), and manganese (Mn) are biologically relevant elements, the use of Zn- and Mn-containing ferrite nanoparticles in biomedical contexts demands careful (geno)toxicity evaluation. In this study, three ferrite nanoparticles – γFe2O3 (FeNPs), Zn0.7Fe2.3O4 (ZnNPs), and Mn0.4Fe2.6O4 (MnNPs) – synthesised through a microwave-assisted polyol route, functionalized with citric acid to improve colloidal stability, were evaluated for their potential (geno)toxic effects in an advanced in vitro 3D cell model, HepG2 spheroids. DNA damage induction was studied using the alkaline comet assay. After 24 and 96 hours of exposure, spheroids were dissociated into a single-cell suspension by collagenase treatment. Each spheroid was incubated in 0.25% trypsin–EDTA for 3 minutes and then gently dissociated into a single-cell suspension using cut pipette tips. After this the comet assay was conducted under conditions described in the Supplement material (Table 1). The cell suspension was combined with 1% low-melting-point (LMP) agarose and layered onto fully frosted slides pre-coated with 1% normal-melting-point (NMP) agarose. Slides were lysed, the nucleoids unwound and electrophoresis preformed, followed by neutralisation of the gells. TEM ferrite-based nanoparticles HepG2 spheroids DNA damage comet assay nanodelci na osnovi ferita HepG2 sferoidi poškodbe DNK kometni test true false false Zenodo Angleški jezik Slovenski jezik Neznano 2026-02-13 03:50:44 2026-08-17 07:56:47 2026-08-18 03:47:56 0000-00-00 00:00:00 2025 0 Genève 0 Nasl. z. nasl. zaslona; Opis vira z dne 30. 1. 2026; Soavtorji: Štern, Alja; Zegura, Bojana; Gallo-Cordova, Alvaro; Morales, Maria del Puerto; Hočevar, Domen; Goya, Gerardo; 1 spletni vir 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-12-11 577.2 10.5281/zenodo.17896231 266870019 https://zenodo.org/records/17896231 1 cbb10e90-0886-11f1-bcbc-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40373 RAZ_Rozman_Iza_2025.zip RAZ_Rozman_Iza_2025.zip 1 7C1396ED576226A2F0FACC58299FFAC9 ba48079ad26f65feec520021ebf26cb1034db4ed5a398f611cfc1ea53ced7916 e38fcc05-2787-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42441 https://doi.org/10.1016/j.biopha.2025.118950 Znanstveni članek Journal article 0 01dadf64-2788-11f1-aa40-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42442 17896231.json 17896231.json 1 E6807D67140D556DDE7EA018FD26FD15 4166922afe55bcf90d21dd9bfdbf06a57d5242572eb93907d1c357f2fb44fc2b 388e60c4-9a00-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49581 Nacionalni inštitut za biologijo Safety of ferrite nanoparticles for biomedical applications: cyto- and genotoxic effects of MxFe3-xO4 (M = Fe, Zn, Mn) in an advanced 3D human hepatic in vitro model 0 0 0