20.500.12556/DiRROS-26994 Tiered genotoxicity testing of enriched river water samples using zebrafish in vitro and in vivo models: a joint Danube Survey 4 case study The increasing complexity of aquatic pollution, dominated by diverse and often uncharacterized chemical mixtures, challenges traditional monitoring approaches. In this study, we assessed the genotoxic potential of surface water samples collected during the Joint Danube Survey 4 (JDS4) using large-volume solid-phase extraction (LVSPE) combined with a comprehensive battery of bioassays. Twenty-three enriched water samples from the Danube River and its major tributaries were evaluated for genotoxicity using a tiered testing strategy comprising the SOS/umuC assay, zebrafish liver (ZFL) cell-based assays (cytotoxicity, comet assay, cell cycle), and zebrafish embryo assays. While no genotoxicity was detected in the prokaryotic SOS/umuC assay, ZFL assays revealed significant DNA damage in 16 out of 23 samples, with notable genotoxicity observed in samples from the middle Danube section. In contrast, no teratogenic effects were observed in zebrafish embryo assays at concentrations up to REF100. These findings demonstrate the superior sensitivity of ZFL cells compared to both prokaryotic and in vivo embryo models. The study also highlights a critical gap in available genotoxicity data for detected substances, emphasizing the need for standardized databases and testing frameworks. Overall, our results support zebrafish-based in vitro assays as effective tools for effect-based monitoring, providing early warnings of genotoxic pollution in complex aquatic environments. ZFL cell line zebrafish embryos cytotoxicity genotoxicity Danube River jetrna celična linija rib cebric (ZFL) embriji rib cebric citotoksičnost genotoksičnost reka Donava true false true Angleški jezik Slovenski jezik Neznano 2026-01-28 12:08:24 2026-01-28 12:08:24 2026-01-31 04:12:26 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: Bojana Žegura, Katja Kološa, Jovana Jovanović Marić, Andrea Novaković, Peter Oswald, Martina Oswaldova, Jaroslav Slobodnik, Nikiforos Alygizakis, Momir Paunović, Marija Ilić, Branka Vuković-Gačić, Stoimir Kolarević; Opis vira z dne 14. 1. 2026; str. 1-11 Vol. 30, [article no.] 100488 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-01-01 577.2 2589-9147 10.1016/j.wroa.2026.100488 264627459 https://www.sciencedirect.com/science/article/pii/S258991472600006X?via%3Dihub 1 b0245ad0-fc39-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39604 1-s2.0-S258991472600006X-mmc1.docx 1-s2.0-S258991472600006X-mmc1.docx 1 86A2892A2D029AB284F2B3C1F1395D6E 30495682179172656e14b7458234d49932b24e6a7a46769ded93240ede13b3a5 0cb09a0c-fc3a-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39606 1-s2.csv 1-s2.csv 1 F60B2A70A9CCC401BC94C00F9F12978D ae8398dc1676c792e125418a54aa725a9dca467a1a7977debfc5ed2a3bfe2e2a 1645d429-fc3a-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39607 1-s2.0-S258991472600006X-mmc3.docx 1-s2.0-S258991472600006X-mmc3.docx 1 7A4B0233BB2260DAFB934484C8392EAC 01631b8b94cfba03c76182517b21e2429700496c07d0b79b562b0657528eb65c 1eb4b7ac-fc3a-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39608 RAZ_Kracun-Kolarevic_Margareta_2026.pdf RAZ_Kracun-Kolarevic_Margareta_2026.pdf 1 416701158B8F88914A7AAA4F62224F00 5a80732496aba2f73686b3a752bf02161a7397b5d2d7324e851085bf1f105567 276ac01a-fc3a-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39610 Nacionalni inštitut za biologijo 0 0 0