20.500.12556/DiRROS-26979 Identification and cultivation of biotechnologically relevant microalgal and cyanobacterial species isolated from Sečovlje salt pans, Slovenia Studies of complex natural environments often focus on either biodiversity or on isolating organisms with specific properties. In this study, we sought to widen this perspective and achieve both. In particular, hypersaline ecosystems, such as the Sečovlje salt pans (Slovenia), are particularly promising sources of novel bioactive compounds, as their microorganisms have evolved adaptations to desiccation and high light intensity stress. We applied shotgun metagenomics to assess microbial biodiversity under low- and high-salinity conditions, complemented by isolation and cultivation of photosynthetic microorganisms. Metagenomic analyses revealed major shifts in community composition with increasing salinity: halophilic Archaea became dominant, while bacterial abundance decreased. Eukaryotic assemblages also changed, with greater representation of salt-tolerant genera such as Dunaliella sp. Numerous additional microorganisms with biotechnological potential were identified. Samples from both petola and brine led to the isolation and cultivation of Dunaliella sp., Tetradesmus obliquus, Tetraselmis sp. and cyanobacteria Phormidium sp./Sodalinema stali, Leptolyngbya sp., and Capilliphycus guerandensis. The newly established cultures are the first collection from this hypersaline environment and provide a foundation for future biodiscovery, production optimization, and sustainable bioprocess development. The methods developed in this study constitute a Toolbox Solution that can be easily replicated in other habitats. culture conditions halophilic organisms hypersaline environment identification of microorganisms shotgun metagenomics photosynthetic microorganisms kultivacijski pogoji halofilni organizmi hiperslano okolje identifikacija mikroorganizmov shotgun metagenomika fotosintetski mikroorganizmi true false true Angleški jezik Slovenski jezik Neznano 2026-01-28 09:42:19 2026-01-28 09:42:20 2026-01-31 04:07:17 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: Petra Tavčar Verdev, Marko Petek, Anna Coll, Daniel Bosch, Marko Dolinar, Viktoriia Komarysta, Neli Glavaš and Ana Rotter; Opis vira z dne 9. 1. 2026; str. 1-28 no. 1, [article no.] 26 Vol. 24 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-01-08 579 1660-3397 10.3390/md24010026 264147203 https://www.mdpi.com/1660-3397/24/1/26 1 45e216e8-fc25-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39575 marinedrugs-24-00026-s001.zip marinedrugs-24-00026-s001.zip 1 B56B286DBAEE3E767C26007961877C79 a35c164deb3567f2bde3b21979e8c0d17a3fca4a9c48af4bd5c07a7d5376fa0b 57696f0a-fc25-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39576 RAZ_Atak_Eylem_2026.pdf RAZ_Atak_Eylem_2026.pdf 1 B992E07A4FE57E0981639BB9A2113384 f7b8d280c4ff3d077947a9e8237e4d26be57d0f98030b862750b50e635b3532e 61aa54ff-fc25-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39577 Nacionalni inštitut za biologijo 0 0 0