20.500.12556/DiRROS-26974 Jasmonic acid and salicylic acid interact to determine spatial regulation of gene expression responses in potato leaf to herbivory by Colorado potato beetle and mechanical wounding We investigated the spatial dynamics of potato (Solanum tuberosum) responses to herbivory and mechanical wounding. We first followed the spatiotemporal response of jasmonic acid (JA) signaling, known to be involved in the response. We generated two potato sensor lines: a JAZ degradation sensor and a downstream multicystatin (MC) transcriptional reporter. Both sensors revealed concentric, locally restricted responses on wounded leaves. Notably, JAdependent gene expression was absent in cells immediately adjacent to the wound, whereas JAZ degradation spread continuously outward from the wound site. This pattern occurred after both herbivore attack and mechanical injury by the needle. To probe the mechanism, a salicylic acid (SA) reporter showed SA accumulation near the wound. Introducing the MC reporter into SA-depleted NahG plants produced a uniform spread of MC expression, confirming that SA attenuates the JA response in proximal cells. Together, these results show that a locally distinct, spatiotemporal SA–JA crosstalk shapes wound responses in potato, extending principles known from pathogen–plant interactions to herbivory and mechanical damage. Colorado potato beetle herbivore hormonal crosstalk jasmonic acid potato salicylic acid wounding koloradski hrošč jasmonska kislina krompir odziv rastlin na ranjenje salicilna kislina true false true Angleški jezik Slovenski jezik Neznano 2026-01-28 08:55:22 2026-01-28 08:55:22 2026-01-31 04:03:36 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 9. 1. 2026; Soavtorji: Tjaša Mahkovec Povalej, Karmen Pogačar, Katja Stare, Maja Zagorščak, Tim Hawkin, Joanne Robson, David Dobnik, Tjaša Lukan, Kristina Gruden; str. 1878-1890 issue 12 Vol. 66 Sep. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-09-24 577.2 1471-9053 10.1093/pcp/pcaf120 264164611 https://academic.oup.com/pcp/article/66/12/1878/8262811?login=false 1 b4807259-fc1e-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39560 pcp-2025-e-00217-file006_pcaf120.pdf pcp-2025-e-00217-file006_pcaf120.pdf 1 EB9DD73F9A20BA5E858220A80E2A81D2 1c4b58a16b1d038bced7d6c4aac6f8ead9e86ca4c246776e2ab5237f8c782217 d8c7c83b-fc1e-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39561 pcp-2025-e-00217-file014_pcaf120.pdf pcp-2025-e-00217-file014_pcaf120.pdf 1 B16E6FE58AFE302E7A97DD3F63C486F2 9a346fc8a8059a670246bba49a94249ca225aa8d08c0fa7d4fe495c0fc567fa4 e2b500b8-fc1e-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39562 data_s1_pcaf120.txt data_s1_pcaf120.txt 1 619B4AF3FBAE33F47628C7BD1EA2D5D5 f4a7f6e3b443f9ea75964a546001b543dd447a43b05395f0c2f48d64ab3315ee eb1e12ce-fc1e-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39563 data_s2_pcaf120.zip data_s2_pcaf120.zip 1 299AB91FDD145FE55D132FC45BF4DF35 c86209c1b355d95040cdd35e66a7cdda340fe31ee70af33c2a1673ab72f73d5c f46fe04a-fc1e-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=39564 Nacionalni inštitut za biologijo 0 0 0