20.500.12556/DiRROS-25545 Epidemiological insights into water and soil-mediatedtransmission of tomato mottle mosaic virus Tomato mottle mosaic virus (ToMMV) poses a growing threat to tomato and pepper crops, yet its epidemiology remains largelyunexplored. This study aimed to investigate water- and soil-mediated transmission of ToMMV under controlled conditions. Theexperiment involved mechanical inoculation of tomato plants, which demonstrated that infectious ToMMV particles remainedviable in water up to 27 weeks at a 10−2 dilution. In hydroponic systems, ToMMV present in the nutrient solution caused systemicinfection in bait plants within 5–17 weeks, depending on the severity of root injury, while infection was not observed in plantswith intact roots even after 38 weeks of exposure. The transmission of the pathogen via irrigation was also confirmed in an ex-periment where seedlings and seed-derived plants grown in substrate (roots were not deliberately injured) were irrigated withToMMV-contaminated water, resulting in infection 6 and 10 weeks after the start of exposure, respectively. The soil-mediatedtransmission process was further verified by planting healthy seedlings and seeds into previously contaminated substrate, re-sulting in infection within a period of 3–18 weeks. These findings emphasise the need for upgrading integrated managementstrategies, including water quality monitoring and reliable, rapid diagnostics, to mitigate the spread of ToMMV in greenhouseproduction systems. To support the development of effective monitoring strategies, the study also assessed the performance oftwo isothermal amplification assays for potential on-site detection of ToMMV in water. A recombinase-polymerase amplification(RPA) assay exhibited sensitivity comparable to that of reverse transcription-quantitative PCR and reliably detected ToMMV incrude water samples without RNA extraction. RPA assay RT- qPCR survival tomato tomato mottle mosaic virus water-linked epidemiology tobamovirusi ToMMV epidemiologija rastni substrat voda true false true Angleški jezik Slovenski jezik Neznano 2026-01-22 13:59:03 2026-01-22 13:59:04 2026-01-24 03:42:56 0000-00-00 00:00:00 2026 0 0 Ostali avtorji: Jakob Brodarič, Irena Bajde, Miha Kitek, Nataša Mehle; Nasl. z nasl. zaslona; Opis vira z dne 20. 1. 2026; str. 1-15 issue 1, [article no.] e70101 Vol. 75 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-11-28 632 1365-3059 10.1111/ppa.70101 265459715 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.70101 1 23e377af-f792-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=38732 RAZ_Vucurovic_Ana_2026.pdf RAZ_Vucurovic_Ana_2026.pdf 1 92421B06E69289348849BE7022150BAB 50cffbd6ecb0b7d75dcbd80a55fc3f5d02d7c20847b67cb4ac1fe23768c2e151 3f26118b-f792-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=38733 Nacionalni inštitut za biologijo 0 0 0